Uptake and processing of the precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase by leucoplasts from the endosperm of developing castor oil seeds.
Uptake and processing of the precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase by leucoplasts from the endosperm of developing castor oil seeds.
复制标题
正在发育的蓖麻油种子的胚乳中的白体对核酮糖 1,5-二磷酸羧化酶小亚基的前体进行吸收和加工。
DOI：
10.1104/pp.81.3.817
复制
发表时间：
1986
期刊：
影响因子：
7.4
通讯作者：
David T. Dennis
中科院分区：
文献类型：
--
作者：
Sheila A. Boyle;S M Hemmingsen;David T. Dennis
Intact leucoplasts from the endosperm of developing castor oil seed were isolated by Percoll density gradient centrifugation. The precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase from pea was synthesized in vitro from hybrid-selected mRNA. Leucoplasts imported this precursor by an ATP-requiring mechanism similar to that described in chloroplasts (AR Grossman et al. 1980 Nature 285: 625-628). The small subunit precursor was processed to a molecular weight that was identical with that of the mature pea small subunit. These results show that leucoplasts, though specialized for fatty acid biosynthesis and not photosynthesis, have a mechanism of protein import similar to that of chloroplasts.
