Chitosan-based cross-linked fluorescent polymer containing aggregation-induced emission fluorogen for cell imaging
Chitosan-based cross-linked fluorescent polymer containing aggregation-induced emission fluorogen for cell imaging
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DOI：
10.1016/j.dyepig.2017.04.055
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发表时间：
2017-08
影响因子：
4.5
通讯作者：
Xie Gaoyi;Chunping Ma;Xiqi Zhang;Hongliang Liu;Liutao Yang;Li Yang;K. Wang;Yen Wei
中科院分区：
文献类型：
--
作者：
Xie Gaoyi;Chunping Ma;Xiqi Zhang;Hongliang Liu;Liutao Yang;Li Yang;K. Wang;Yen Wei
通过羧甲基壳聚糖与带有两个环氧乙烷的AIE单体的开环聚合，制备了含有聚集诱导发射(AIE)荧光的壳聚糖基交联荧光聚合物(Ch-EP3)。通过1H NMR、FT-IR和X射线光电子能谱对其进行表征并证明其成功合成。壳聚糖组分的引入赋予了Ch-EP3两亲性，Ch-EP3可以组装成纳米颗粒，易于分散在水中形成荧光聚合物纳米颗粒(FPNs)。动态光散射和透射电子显微镜结果表明 FPN 的尺寸分布为 260 ± 12 nm，呈球形。所制备的 FPNs 的荧光光谱显示出强烈的绿色荧光，荧光量子产率为 35%。 Ch-EP3FPN 在高度稀释的溶液中进一步表现出优异的颗粒稳定性，临界胶束浓度低至 0.063 mg mL−1。合成的 FPN 在 60 分钟的紫外光照射下也表现出高光稳定性。细胞毒性评估证明，不同浓度的 Ch-EP3FPN 孵育 8 小时或 24 小时后，细胞具有较高的活力，并且具有高度的细胞成像生物相容性。利用高性能Ch-EP3FPNs的高水分散性、良好的颗粒稳定性和优异的光稳定性等优势，这项工作将激发更多方法来制备用于生物医学应用的新型交联生物相容性荧光聚合物。
A chitosan-based cross-linked fluorescent polymer (Ch-EP3) containing aggregation-induced emission (AIE) fluorogen was prepared through ring-opening polymerization of carboxymethyl chitosan and an AIE monomer with two ethylene oxide groups.1H NMR, FT-IR, and X-ray photoelectron spectra were conducted to characterize and prove its successful synthesis. The introduction of chitosan component endowedCh-EP3with amphiphilic property, andCh-EP3could assemble into nanoparticles, which were facilely dispersed in water to form fluorescent polymeric nanoparticles (FPNs). The dynamic light scattering and transmission electron microscopy results indicated the size distribution of the FPNs was 260 ± 12 nm with spherical shape. Fluorescent spectrum of the prepared FPNs demonstrated intense green fluorescence with high fluorescence quantum yield of 35%. TheCh-EP3FPNs further showed excellent particle stability at highly dilute solution with low critical micelle concentration of 0.063 mg mL−1. The synthesized FPNs also indicated high photostability under UV light irradiation for a period of 60 min. The cytotoxicity evaluation proved high cell viability after incubation of theCh-EP3FPNs at different concentrations for a period of 8 h or 24 h, and demonstrated highly biocompatible for cell imaging. Taking advantage of the high-performanceCh-EP3FPNs, such as, high water dispersibility, good particle stability, and excellent photostability, this work would inspire more approaches to prepare novel cross-linked biocompatible fluorescent polymers for biomedical application.