Highly sensitive fluorescence-linked immunosorbent assay based on aggregation-induced emission luminogens incorporated nanobeads.
Highly sensitive fluorescence-linked immunosorbent assay based on aggregation-induced emission luminogens incorporated nanobeads.
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DOI：
10.1016/j.bios.2019.111912
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发表时间：
2019-11
影响因子：
12.6
通讯作者：
Weijie Wu;Mengfei Shen;Xinyi Liu;Lisong Shen;X. Ke;Wanwan Li
中科院分区：
文献类型：
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作者：
Weijie Wu;Mengfei Shen;Xinyi Liu;Lisong Shen;X. Ke;Wanwan Li
由于其独特的光学特性，聚集诱导发射发光物质在各种生物传感器的开发中得到了广泛的应用。然而，产生合适的荧光信号以构建基于aiegens的免疫分析平台的主要挑战是激活AIE过程所需的复杂表面修饰和额外的化学反应。本文报道了一种新的基于AIEgens纳米微球的荧光联免疫吸附测定（FLISA）平台，其中荧光标记为六苯基硅酮（HPS）纳米微球，该微球通过Shirasu多孔玻璃（SPG）膜乳化法合成，无需任何预处理即可提供强烈的直接荧光信号。此外，基于粒子的信号放大效应使该平台显著提高了癌胚抗原（CEA）定量的检测灵敏度。与使用r -植红蛋白（PE）或商用绿色量子点纳米粒作为荧光标记的FLISA相比，基于AIEgens纳米粒的FLISA平台的检测灵敏度分别提高了45倍和12倍。应用AIEgens纳米微球FLISA免疫分析平台分析人血清样品的临床验证实验结果与临床金标准方法电化学发光免疫分析（ECLIA）结果一致。HPS纳米球具有较强的抗光漂白能力和优异的荧光稳定性，无需遮光，提高了效率，使操作条件更加舒适。因此，这个基于AIEgens纳米珠的FLISA平台，具有直接荧光信号产生和显著的信号放大等吸引人的特点，为AIEgens在生物传感器和临床诊断中的应用开辟了一条新的、通用的途径。
Aggregation-induced emission luminogens (AIEgens) have attracted considerable interest for application towards the development of various biosensors due to their unique optical properties. However, the major challenge associated with generating a suitable fluorescent signal for constructing an AIEgens-based immunoassay platform, is the complex surface modification and additional chemical reaction required to activate the AIE process. This work reports a novel AIEgens nanobeads-based fluorescence-linked immunosorbent assay (FLISA) platform wherein the fluorescent labels are hexaphenylsilole (HPS) nanobeads, which were synthesized through Shirasu porous glass (SPG) membrane emulsification method and could provide a strong, direct fluorescent signal without any pretreatment. Moreover, the particle-based signal amplification effect affords this platform significantly improved detection sensitivity for carcinoembryonic antigen (CEA) quantitation. Compared to FLISA which uses R-phycoerythrin (PE) or commercial green QDs nanobeads as fluorescent labels, this AIEgens nanobeads-based FLISA platform exhibits detection sensitivity improved up to 45-fold and 12-fold, respectively. Clinical validation experiments applying this AIEgens nanobeads-based FLISA immunoassay platform to analyze human serum samples produce results consistent with those obtained by the clinical gold-standard method, electrochemiluminescence immunoassay (ECLIA). The strong photobleaching resistance and excellent fluorescent stability of the HPS nanobeads negate the need for light shielding, which improves the efficiency and makes the operating conditions more comfortable. Thus, this AIEgens nanobeads-based FLISA platform, with attractive features including direct fluorescent signal generation and significant signal amplification, creates a new, versatile route for the application of AIEgens in biosensors and clinical diagnosis.