Silver nanoparticle based surface enhanced Raman scattering spectroscopy of diabetic and normal rat pancreatic tissue under near-infrared laser excitation
Silver nanoparticle based surface enhanced Raman scattering spectroscopy of diabetic and normal rat pancreatic tissue under near-infrared laser excitation
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近红外激光激发下糖尿病和正常大鼠胰腺组织的基于银纳米颗粒的表面增强拉曼散射光谱
DOI：
10.1088/1612-2011/10/4/045603
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发表时间：
2013-04-01
影响因子：
1.7
通讯作者：
Chen, R.
中科院分区：
文献类型：
--
作者：
Huang, H.;Shi, H.;Chen, R.
利用高空间分辨率的纳米银颗粒对大鼠胰腺组织进行近红外表面增强拉曼散射(SERS)，获取胰腺组织的生物化学信息。使用雷尼绍微拉曼系统，可以在10 S内获得来自胰腺组织和银纳米颗粒混合物的高质量表面增强拉曼信号。胰腺组织的显著SERS条带被归类为已知的分子振动，如DNA碱基、RNA碱基、蛋白质和脂类的振动。糖尿病大鼠和正常大鼠胰腺组织的不同组织结构在表面增强拉曼散射光谱中具有一定的特征。这项探索性研究展示了使用SERS成像在冰冻切片上区分糖尿病和正常胰腺组织的巨大潜力，而不需要使用功能化结合部位的染料标记。
This paper presents the use of high spatial resolution silver nanoparticle based near-infrared surface enhanced Raman scattering (SERS) from rat pancreatic tissue to obtain biochrmical information about the tissue. A high quality SERS signal from a mixture of pancreatic tissues and silver nanoparticles can be obtained within 10 s using a Renishaw micro-Raman system. Prominent SERS bands of pancreatic tissue were assigned to known molecular vibrations, such as the vibrations of DNA bases, RNA bases, proteins and lipids. Different tissue structures of diabetic and normal rat pancreatic tissues have characteristic features in SERS spectra. This exploratory study demonstrated great potential for using SERS imaging to distinguish diabetic and normal pancreatic tissues on frozen sections without using dye labeling of functionalized binding sites.