SINGLE-CHANNEL PROPERTIES OF THE 5-HT3 SUBTYPE OF SEROTONIN RECEPTOR IN PRIMARY CULTURES OF RODENT HIPPOCAMPUS
SINGLE-CHANNEL PROPERTIES OF THE 5-HT3 SUBTYPE OF SEROTONIN RECEPTOR IN PRIMARY CULTURES OF RODENT HIPPOCAMPUS
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DOI：
10.1016/0304-3940(94)90004-3
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发表时间：
1994-06-20
影响因子：
2.5
通讯作者：
SURPRENANT, A
中科院分区：
文献类型：
--
作者：
JONES, KA;SURPRENANT, A
从大鼠和小鼠海马神经元原代培养中获得全细胞和外向膜片钳记录。5-羟色胺（5-HT）在大约5%的神经元中诱发了短潜伏期快速内向电流。这些电流在0 mV附近逆转，表现为向内整流，并被5-HT、受体拮抗剂ICS 205-930、昂丹司酮和tubocurare抑制。5-羟色胺在24个神经元膜斑块中激活了14个单通道电流，显示了5-羟色胺激活的全细胞电流；在-100 mV时，通道开口的平均振幅为0.95 +/- 0.09 pA。在-80和-160 mV下测得的弦导分别为8.3和10.5 pS。tubocurare （1 μ M）、ICS 205-930 （30 nM）和ondansetron （100 nM）可逆阻断5- ht诱导的单一电流。因此，海马神经元中5-HT3受体的单通道特性与外周神经元中的相似，并不仅仅表现出神经母细胞瘤和神经母细胞瘤衍生的克隆5-HT3受体的亚小管传导特性。
Whole cell and outside-out patch clamp recordings were obtained from primary cultures of rat and mouse hippocampal neurons. Serotonin (5-HT) evoked short-latency fast inward currents in about 5% of neurons. These currents reversed near 0 mV, showed inward rectification, and were inhibited by the 5-HT, receptor antagonists ICS 205-930, ondansetron and tubocurare. 5-HT activated single channel currents in 14 of 24 membrane patches obtained from neurons which showed 5-HT3-activated whole cell currents; mean amplitude of channel openings was 0.95 +/- 0.09 pA at -100 mV. Chord conductances measured at -80 and -160 mV were 8.3 and 10.5 pS, respectively. 5-HT-induced unitary currents were blocked reversibly by tubocurare (1 mu M), ICS 205-930 (30 nM) and ondansetron (100 nM). Thus, single-channel properties of 5-HT3 receptors in hippocampal neurons are similar to those present in peripheral neurons and do not exhibit solely the sub-picosiemen conductance characteristic of the neuroblastoma and neuroblastoma-derived cloned 5-HT3 receptor.