REVERSIBLE INHIBITION OF INTERCELLULAR JUNCTIONAL COMMUNICATION BY GLYCYRRHETINIC ACID
REVERSIBLE INHIBITION OF INTERCELLULAR JUNCTIONAL COMMUNICATION BY GLYCYRRHETINIC ACID
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DOI：
10.1016/0006-291x(86)90522-x
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发表时间：
1986-01-14
影响因子：
3.1
通讯作者：
HARLEY, EH
中科院分区：
文献类型：
--
作者：
DAVIDSON, JS;BAUMGARTEN, IM;HARLEY, EH
使用代谢合作，在共培养物中的琥珀酸合成酶缺陷和琥珀酸裂解酶缺陷的人成纤维细胞测定细胞间的缝隙连接通讯。18-α-发现大黄酸（阿加）在低至2 μ M的浓度下抑制通讯超过95%。浓度高达100 μ M在2小时内没有细胞毒性。交流抑制是快速发作，很容易逆转。在阿加存在下培养20天的细胞中，通讯保持连续但可逆的阻断。相关化合物18-β-大黄酸和甘珀酸也引起通讯抑制。这种作用可能不是通过盐皮质激素或糖皮质激素受体介导的，因为醛固酮和糖皮质激素对交流没有影响。因此，阿加具有在细胞间连接通讯研究中有用的抑制剂的特性。
Intercellular gap-junctional communication was measured using metabolic co-operation in co-cultures of argininosuccinate synthetase-deficient and argininosuccinate lyase-deficient human fibroblasts. 18-.alpha.-glycyrrhetinic acid(AGA) was found to inhibit communication by more than 95% at concentrations as low as 2 .mu.M. Concentrations up to 100 .mu.M were not cytotoxic over a period of 2 hours. Communication inhibition was of rapid onset and was readily reversible. Communication remained continuously yet reversibly blocked in cells cultured in the presence of AGA for 20 days. The related compounds 18-.beta.-glycyrrhetinic acid and carbenoxolone also caused communication inhibition. The effect is probably not mediated via mineralocorticoid or glucocorticoid receptors since aldosterone and glucocorticoids had no effect on communication. AGA thus has properties of a useful inhibitor in the study of intercellular junctional communication.