Postnatal changes of nicotinic acetylcholine receptor alpha 2, alpha 3, alpha 4, alpha 7 and beta 2 subunits genes expression in rat brain.
Postnatal changes of nicotinic acetylcholine receptor alpha 2, alpha 3, alpha 4, alpha 7 and beta 2 subunits genes expression in rat brain.
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大鼠脑中烟碱乙酰胆碱受体α2、α3、α4、α7和β2亚基基因表达的出生后变化。
DOI：
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发表时间：
1998
影响因子：
1.8
通讯作者：
A. Nordberg
中科院分区：
文献类型：
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作者：
X. Zhang;C. Liu;H. Miao;Z. Gong;A. Nordberg
用核糖核酸酶保护法研究了生后大鼠脑内烟碱乙酰胆碱受体（nAChR）α 2、α 3、α 4、α 7和β 2亚基mRNA的变化。观察到多种发育模式：（1）出生后最初几周内的瞬时表达;海马和脑干中的α 2，纹状体、小脑和皮质中的α 3，海马、纹状体和小脑中的α 4，小脑中的α 7和纹状体中的β 2。(2)在整个发育过程中持续表达; α 2和α 3在丘脑中，α 4在皮质、丘脑和脑干中，α 7在丘脑和脑干中，β 2在除纹状体外的所有脑区中。(3)在整个发育过程中未检测到;皮质、纹状体和小脑中的α 2。(4)随着年龄的增长而增加;皮质和海马体中的α 7。(5)钟形发育;纹状体中的α 7。用受体结合分析法研究了大鼠生后不同脑区nAChR亚型的变化。[~ 3 H]地棘蛙素和（-）-[~ 3 H]烟碱结合位点在各脑区的发育模式相似，但与[~ 3 H] α-银环蛇毒素结合位点的发育模式不同。[~ 3 H] α-银环蛇毒素、[~ 3 H]地棘蛙素和（-）-[~ 3 H]烟碱结合位点的发育模式与相应的亚基mRNA之间没有明显的相关性。这些结果表明，发育期大鼠脑内nAChRs亚单位基因表达的变化涉及多种机制。
Postnatal changes of nicotinic acetylcholine receptor (nAChR) alpha 2, alpha 3, alpha 4, alpha 7 and beta 2 subunits mRNAs were investigated in rat brain using ribonuclease protection assay. Multiple developmental patterns were observed: (1) transient expression during the first few postnatal weeks; alpha 2 in the hippocampus and brain stem, alpha 3 in the striatum, cerebellum and cortex, alpha 4 in the hippocampus, striatum and cerebellum, alpha 7 in the cerebellum and beta 2 in the striatum. (2) Constant expression across development; alpha 2 and alpha 3 in the thalamus, alpha 4 in the cortex, thalamus and brain stem, alpha 7 in the thalamus and brain stem and beta 2 in all brain regions except striatum. (3) Non-detection across development; alpha 2 in the cortex, striatum and cerebellum. (4) Increase with age; alpha 7 in the cortex and hippocampus. (5) Bell-shaped development; alpha 7 in the striatum. Postnatal changes of nAChR isoforms in different brain regions of rat were investigated by receptor binding assays. The developmental patterns of [3H]epibatidine and (-)-[3H]nicotine binding sites were similar to each other in each brain region, but different from that of [3H] alpha-bungarotoxin binding sites. No obvious correlation was observed between the developmental patterns of [3H] alpha-bungarotoxin, [3H]epibatidine and (-)-[3H]nicotine binding sites and corresponding subunits mRNAs. These results indicate that multiple mechanisms are involved in changes of gene expression of nAChRs subunits in the brain of developing rats.