RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus
RNA sequencing analyses of gene expressions in a canine macrophages cell line DH82 infected with canine distemper virus
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感染犬瘟热病毒的犬巨噬细胞系DH82基因表达的RNA测序分析
DOI：
10.1016/j.meegid.2020.104206
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发表时间：
2020-06-01
影响因子：
3.2
通讯作者：
Xue，Xianghong
中科院分区：
文献类型：
--
作者：
Zheng，Xuexing;Zhu，Yelei;Xue，Xianghong
包括MeV和CDV在内的毒性麻疹病毒感染引起严重的免疫抑制和白细胞减少，而减毒疫苗株则产生保护性宿主免疫应答。然而，宿主抗病毒反应的详细分子基础的特点很差。为了更好地了解弱毒疫苗与宿主抗病毒反应之间的相互作用，采用转录组学方法研究了CDV-11感染犬巨噬细胞源性细胞系DH 82细胞后的基因表达变化，并采用定量RT-PCR方法对部分结果进行了验证。结果显示，在感染后48小时，在CDV感染的巨噬细胞DH 82中，372个基因显著上调（p <0.01），119个基因显著下调（p<0.01）。显著上调（p<0.01）基因的富集功能与干扰素刺激基因（ISG）、趋化因子基因和促炎因子基因密切相关。差异表达基因（DEG）的基因本体和通路分析显示，在CDV感染的DH 82细胞中，最重要的参与通路是NF-κB和TNF信号通路、IFN-γ-细胞因子受体相互作用和病原体相关分子模式（PAMPs），如Toll样、RIG-I样和NOD样受体信号通路。因此，研究结果表明，模式识别受体（PRRs）可能介导宿主先天性和保护性抗病毒免疫反应在CDV-11感染的DH 82细胞。
Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p< .01) and 119 genes were significantly down-regulated (p< .01) in CDV-infected macrophages DH82 at 48 h p.i.. The enriched functions of the significantly up-regulated (p< .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells.