BCSG1 methylation status and BCSG1 expression in breast tissues derived from Chinese women with breast cancer
BCSG1 methylation status and BCSG1 expression in breast tissues derived from Chinese women with breast cancer
复制标题
中国乳腺癌女性乳腺组织中 BCSG1 甲基化状态及 BCSG1 表达
DOI：
10.1159/000139125
复制
发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Sun, Yu-Jie
中科院分区：
文献类型：
--
作者：
Gu, Yu-Mei;Tan, Jian-Xin;Sun, Yu-Jie
目的：目的探讨中国人乳腺癌组织中BCSG 1基因表达的甲基化调控。研究方法：使用甲基化特异性PCR分析广泛检测乳腺癌和匹配的非肿瘤性邻近和良性病变组织中BCSG 1基因外显子1的甲基化状态。通过实时PCR测定相应的mRNA表达水平。结果如下：我们发现：（1）BCSG 1基因在所有乳腺组织中普遍去甲基化，无论组织状态如何，尽管50-60%的样品也显示甲基化产物;（2）DNA甲基化与BCSG 1基因在肿瘤组织中的表达相关，但在非肿瘤性邻近组织和良性组织中不相关;（3）乳腺肿瘤组织表达的BCSG 1低于非肿瘤性邻近组织。结论：我们的数据揭示了BCSG 1基因在中国患者乳腺组织中独特的表达模式和甲基化状态，并表明BCSG 1调控的甲基化状态和机制可能与种族背景密切相关。版权所有（c）2008 S. Karger AG，巴塞尔。
Objective: To investigate the role of DNA methylation in the regulation of BCSG1 gene expression in breast tissues derived from Chinese women with breast cancer. Methods: The methylation status of exon 1 of the BCSG1 gene in breast cancer, and matched non-neoplastic adjacent and benign lesion tissues was extensively examined using methylation-specific PCR analysis. Corresponding mRNA expression levels were determined by real-time PCR. Results: We found: (1) the BCSG1 gene was universally demethylated in all breast tissues regardless of the tissue state, although 50-60% of the samples displayed methylated products as well; (2) DNA methylation correlated to the expression of the BCSG1 gene in tumor tissues, but not in non-neoplastic adjacent and benign tissues; (3) breast tumor tissues expressed lower BCSG1 than non-neoplastic adjacent tissues. Conclusions: Our data revealed a unique expression pattern and methylation status of the BCSG1 gene in breast tissues derived from Chinese patients and suggested both methylation status and mechanisms underlying BCSG1 regulation might be closely related to the racial background. Copyright (c) 2008 S. Karger AG, Basel.