Growth of human digestive-tumour xenografts in athymic nude rats.
Growth of human digestive-tumour xenografts in athymic nude rats.
复制标题
人类消化肿瘤异种移植物在无胸腺裸鼠中的生长。
作者：
G. Davies;D. Duke;A. Grant;S. A. Kelly;J. Hermon
无瘤裸鼠RNU/RNU已被建立为接受人消化系肿瘤移植瘤的体内模型。我们报告了7/12(58%)的消化道癌患者的原代移植成功。21/25(84%)的胰腺肿瘤来自细胞培养的胰腺外分泌腺癌(GER)，其中2例已在裸鼠体内成功传代。同时将这些肿瘤移植到裸鼠体内，结果几乎是相同的。将裸鼠的一只结肠和一只胰腺移植到裸鼠体内，再移植回裸鼠体内，均可提高移植成功率。建立原发肿瘤生长的关键期通常为28-42天。异种移植保持了原始外植体或原始肿瘤的组织和细胞学特征。该细胞系的核型在实体瘤中也保持不变。三种小鼠肿瘤作为异种移植瘤成功生长。尽管它们免疫功能低下，但与饲养在隔离器中的动物相比，这项研究中的大鼠在常规条件下没有表现出发病率或死亡率的增加。裸鼠将成为建立和维持人类消化系统肿瘤以及外科和系列血清学研究的有价值的补充工具。
The athymic nude rat rnu/rnu has been established as an in vivo model for the acceptance of human digestive-tumour xenografts. We report the successful xenografting of 7/12 (58%) primary explants from patients with digestive cancer. Successful xenografting also occurred in 21/25 (84%) pancreatic tumours derived from a pancreatic exocrine adenocarcinoma (GER) maintained in cell culture, 2 of those have been successfully passaged in nude rats. The simultaneous implantation of these tumours into nude mice led to an almost identical take rate. Passage of one colonic and one pancreatic xenograft from nude rats into nude mice, and transplantation back into nude rats, increased the take rates. The critical period for the establishment of primary tumour growth was usually 28-42 days. The xenografts maintained histological and cytological characteristics of the primary explants or of the original tumour from which the cell line derived. The karyotype of the cell line was also maintained in the solid tumour. Three murine tumours were successfully grown as xenografts. Despite their immunoincompetence, the rats in this study showed no increased morbidity or mortality when kept in conventional conditions, compared with animals housed in isolators. The athymic nude rat will become a valuable complementary tool to the nude mouse for the establishment and maintenance of human digestive tumours and for surgical and serial serological studies.