Circular RNA Circ_0000064 promotes the proliferation and fibrosis of mesangial cells via miR-143 in diabetic nephropathy
Circular RNA Circ_0000064 promotes the proliferation and fibrosis of mesangial cells via miR-143 in diabetic nephropathy
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环状RNA Circ_0000064通过miR-143促进糖尿病肾病肾小球系膜细胞的增殖和纤维化
DOI：
10.1016/j.gene.2020.144952
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发表时间：
2020-10-01
期刊：
影响因子：
3.5
通讯作者：
Huang, Shan
中科院分区：
文献类型：
--
作者：
Ge, Xiaoxu;Xi, Liuqing;Huang, Shan
糖尿病肾病（Diabetic nephropathy， DN）是糖尿病患者最常见的微血管并发症之一，可导致慢性肾功能衰竭和终末期肾病。包括环状rna （circRNAs）和微rna （miRNAs）在内的非编码rna被广泛报道在包括DN在内的许多人类疾病中发挥关键作用。本研究旨在探讨circ_0000064在糖尿病肾病进展中的作用。结果显示circ_0000064显著促进DN系膜细胞增殖，加重纤维化。在随后的机制研究中，我们发现circ_0000064通过靶向微RNA miR-143参与了这一过程。miR-143的抑制显著逆转了circ_0000064沉默对DN的影响。总之，我们证明了circ_0000064在DN中的调节作用，并阐明了circ_0000064通过靶向miR-143在DN中发挥作用。Circ_0000064和miR-143也显示出作为DN生物标志物的潜力。
Diabetic nephropathy (DN) as one of the most frequent microvascular complications of diabetic patients causes chronic renal failure and end-stage renal disease. Noncoding RNAs including circular RNAs (circRNAs) and micro RNAs (miRNAs) were widely reported to play a critical role in numerous human diseases including DN. This research was designed to investigate the role of circ_0000064 in diabetic nephropathy progression. The results showed that circ_0000064 significantly promoted mesangial cells proliferation and aggravated fibrosis in DN. In the subsequent mechanism investigation, we found that circ_0000064 was involved in this process by targeting micro RNA miR-143. The inhibition of miR-143 significantly reverses the effect of circ_0000064 silencing on DN. In conclusion, we demonstrated the regulatory role of circ_0000064 in DN and clarified that circ_0000064 play a role in DN via targeting miR-143. Circ_0000064 and miR-143 also showed the potential as a biomarker for DN.