SPECIES-SPECIFIC DIVERSITY AMONG SIMIAN IMMUNODEFICIENCY VIRUSES FROM AFRICAN-GREEN MONKEYS
SPECIES-SPECIFIC DIVERSITY AMONG SIMIAN IMMUNODEFICIENCY VIRUSES FROM AFRICAN-GREEN MONKEYS
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DOI：
10.1128/jvi.65.6.2816-2828.1991
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发表时间：
1991-06-01
影响因子：
5.4
通讯作者：
HAHN, BH
中科院分区：
文献类型：
--
作者：
ALLAN, JS;SHORT, M;HAHN, BH
大多数非洲野生猴类物种中猴免疫缺陷病毒（SIV）感染的流行率、自然史和遗传特征目前尚不清楚，但这些信息对于阐明它们的起源以及与其他猴类和人类免疫缺陷病毒的关系至关重要。在这项研究中，综合运用了经典方法和分子方法来鉴定和表征来自西非绿猴（Cercopithecus sabaeus）的SIV分离株（SIVagm分离株）。从野生捕获的西非绿猴中分离出了4种SIVagm病毒，并对其进行了生物学和分子学分析。通过巢式聚合酶链反应，直接从未经培养的外周血单核细胞中扩增、克隆并测序了一个279bp的聚合酶片段。结果表明，西非绿猴自然感染了与东非SIVagm分离株密切相关的SIV。然而，观察到的结构、抗原和遗传差异强烈表明，西非绿猴病毒构成了SIVagm的一个系统发育上独特的亚群。这些发现支持了我们之前的假设，即SIVagm病毒可能随着其非洲绿猴宿主的进化和分化而进化和分化。此外，这项研究描述了一种基于聚合酶链反应的方法，该方法能够直接从原代猴组织中鉴定和分子分析不同的SIV毒株。这种不依赖病毒分离方法的途径，应该会有助于未来旨在阐明非洲野生绿猴种群中SIV的起源和自然史的研究。
The prevalence, natural history, and genetic characteristics of simian immunodeficiency virus (SIV) infections in most feral African monkey species are presently unknown, yet this information is essential to elucidate their origin and relationship to other simian and human immunodeficiency viruses. In this study, a combination of classical and molecular approaches were used to identify and characterize SIV isolates from West African green monkeys (Cercopithecus sabaeus) (SIVagm isolates). Four SIVagm viruses from wild-caught West African green monkeys were isolated and analyzed biologically and molecularly. Amplification, cloning, and sequencing of a 279-bp polymerase fragment directly from uncultured peripheral blood mononuclear cells was facilitated by the use of nested polymerase chain reaction. The results indicated that West African green monkeys are naturally infected with SIVs which are closely related to East African SIVagm isolates. However, structural, antigenic, and genetic differences were observed which strongly suggest that the West African green monkey viruses comprise a phylogenetically distinct subgroup of SIVagm. These findings support our previous hypothesis that SIVagm viruses may have evolved and diverged coincident with the evolution and divergence of their African green monkey host. In addition, this study describes a polymerase chain reaction-based approach that allows the identification and molecular analysis of divergent SIV strains directly from primary monkey tissue. This approach, which does not depend on virus isolation methods, should facilitate future studies aimed at elucidating the origins and natural history of SIVs in feral African green monkey populations.