Production of human chorionic gonadotropin and its subunits by human tumors growing in nude mice.
Production of human chorionic gonadotropin and its subunits by human tumors growing in nude mice.
复制标题
裸鼠体内生长的人类肿瘤产生人绒毛膜促性腺激素及其亚基。
作者：
R. W. Ruddon;A. Bryan;K. Meade;V. Pollack
对在裸鼠体内生长的人类肿瘤产生和分泌人绒毛膜促性腺激素（HCG）及其亚单位的情况进行了研究。在裸鼠体内生长的JAR绒毛膜癌细胞既能产生游离的α亚单位，也能产生完整的HCG，但与在培养条件下这些细胞产生的HCG亚单位相比，体内产生的游离α亚单位相对于完整HCG的量有所减少。在培养条件下只产生游离α亚单位的细胞系（HeLa宫颈癌、ChaGo支气管癌和BT - 20乳腺癌）在体内仍然主要产生游离α亚单位，但从携带HeLa或ChaGo肿瘤的小鼠收集的24小时尿液中可检测到少量的HCG - β/HCG。源自多形性胶质母细胞瘤的CBT细胞在体内既能产生α亚单位，也能产生HCG - β/HCG。这与CBT细胞在培养条件下产生HCG亚单位的模式明显不同，因为培养的CBT细胞只产生游离的β亚单位，既不合成游离的α亚单位，也不合成完整的HCG。因此，对于在裸鼠体内生长的人类肿瘤，与培养细胞所观察到的模式相比，似乎更倾向于产生完整的HCG，而游离亚单位的产生减少。
The production and secretion of human chorionic gonadotropin (HCG) and its subunits by human tumors growing in nude mice have been examined. JAR choriocarcinoma cells growing in nude mice produce both free alpha subunit and complete HCG, but there is a decrease in the amount of free alpha subunit relative to complete HCG produced in vivo compared to HCG subunit production by these cells growing in culture. Cell lines that produce only free alpha subunit in culture (HeLa cervical carcinoma, ChaGo bronchogenic carcinoma, and BT-20 breast carcinoma) continue to produce primarily free alpha subunit in vivo, but a small amount of HCG-beta/HCG is detectable in the 24-hr urine collected from mice bearing HeLa or ChaGo tumors. CBT cells derived from a glioblastoma multiforme produce both alpha and HCG-beta/HCG in vivo. This represents a distinct shift from the pattern of HCG subunit production by CBT cells in culture because cultured CBT cells produce only free beta subunit and do not synthesize either free alpha or complete HCG. Thus, for human tumors growing in nude mice, there appears to be a shift toward more complete HCG production and a decrease in free subunit production as compared to the pattern observed for cultured cells.