Laser phase plate for transmission electron microscopy
Laser phase plate for transmission electron microscopy
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DOI：
10.1038/s41592-019-0552-2
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发表时间：
2019-10-01
期刊：
影响因子：
48
通讯作者：
Mueller, Holger
中科院分区：
文献类型：
--
作者：
Schwartz, Osip;Axelrod, Jeremy J.;Mueller, Holger
快速冷冻生物样本的透射电子显微镜（TEM）或冷冻EM将受益于用于聚焦相衬成像的相位板的开发。已经研究了几种类型的相位板，但是所有这些装置的快速静电充电阻碍了这些努力。在这里，我们演示了电子相位操纵与高强度连续波激光束，并使用它作为TEM的相位板。我们通过对非晶碳膜成像来演示激光相位板。激光相位板提供稳定且可调谐的相移，而没有静电充电或不需要的电子散射。这些结果表明未染色的生物大分子和细胞的剂量有效成像的可能性。
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Here, we demonstrate electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. We demonstrate the laser phase plate by imaging an amorphous carbon film. The laser phase plate provides a stable and tunable phase shift without electrostatic charging or unwanted electron scattering. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells.