Quantitative analysis of Ichthyobodo salmonis an ectoparasitic flagellate infecting juvenile chum salmon Oncorhynchus keta in hatcheries
Quantitative analysis of Ichthyobodo salmonis an ectoparasitic flagellate infecting juvenile chum salmon Oncorhynchus keta in hatcheries
复制标题
孵化场中感染幼年鲑鱼 Oncorhynchus keta 的外寄生鞭毛虫的定量分析
DOI：
10.1007/s12562-016-1055-z
复制
发表时间：
2017
影响因子：
1.9
通讯作者：
Ueda H
中科院分区：
文献类型：
--
作者：
Mizuno S;Urawa S;Miyamoto M;Hatakeyama M;Koide N;Ueda H
由外寄生鞭毛虫引起的鱼体病鱼体病是在日本北方孵化场饲养的幼鲑Oncorhynchus ketared死亡的一个重要原因。本研究建立了实时定量聚合酶链反应（qPCR）检测I.鲑鱼核糖体DNA（rDNA），使用SYBR绿色。该检测允许监测寄生虫感染，用于流行病学研究和控制孵化场中的鱼鳞病。qPCR在1.0~1.0 × 108rDNA拷贝/μ l范围内具有较高的重复性。寄生虫数量与I含量呈显著正相关。鲑鱼rDNA。使用qPCR检测的调查表明，I. 87个孵化场中有23个存在鲑鱼感染;寄生虫负载估计在50至750个寄生虫/克幼鱼体重之间。这些结果表明，我们的qPCR测定能够调查在孵化场饲养的幼鲑鱼感染I。鲑鱼
Ichthyobodosis caused by the ectoparasitic flagellateIchthyobodo salmonisis a significant cause of mortality in juvenile chum salmonOncorhynchus ketareared in hatcheries of northern Japan. The present study established a real-time quantitative polymerase chain reaction assay (qPCR) ofI. salmonisribosomal DNA (rDNA) using SYBR Green. This assay allows monitoring of parasite infections for the epidemiological study and control of ichthyobodosis in hatcheries. qPCR showed high reproducibility for measurements between 1.0 and 1.0 × 108rDNA copy/μl. There was a significant positive correlation between the number of parasites and the amount ofI. salmonisrDNA. A survey using the qPCR assay indicated that infection byI. salmoniswas present in 23 of 87 hatcheries; parasite loads were estimated to be between 50 and 750 parasites/g juvenile body weight. These results demonstrate that our qPCR assay enables the surveying of juvenile chum salmon reared in hatcheries for infection byI. salmonis.