Erythropietic colonies in cultures of human marrow.
Erythropietic colonies in cultures of human marrow.
复制标题
人类骨髓培养物中的红细胞集落。
作者：
A. Tepperman;J. Curtis;E. McCulloch
由Stephenson和Axelrad最初设计的用于小鼠细胞促红细胞生成素依赖性红细胞集落形成（CFU-E）的细胞培养方法已适用于人骨髓。来自人骨髓的红细胞生成菌落含有8至150个细胞，孵育7-9天后达到最大生长。较大菌落的祖细胞与人类CFU-E总池的不同之处在于，它们的平均沉降速度较慢，对促红细胞生成素的需求较大。该测定是定量的，用于测定人红细胞祖细胞的辐射敏感性。生存曲线参数为：D = 113±7.8,n = 1.5±0.15。
The cell culture method originally devised by Stephenson and Axelrad for erythropoietin-dependent erythropoietic colony formation (CFU-E) by mouse cells has been adapted to human marrow. Erythropoietic colonies from human marrow contain between eight and 150 cells and reach maximum growth after 7-9 days of incubation. Progenitors of larger colonies differ from the total pool of human CFU-E in that they have a slower mean sedimentation velocity and a greater erythropoietin requirement. The assay is quantitative and was used to determine the radiation sensitivity of human erythropoietic progenitors. The parameters of the survival curve are: D o = 113 ± 7.8, n = 1.5 ± 0.15.