Calcineurin is required for Candida albicans to survive calcium stress in serum
Calcineurin is required for Candida albicans to survive calcium stress in serum
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DOI：
10.1128/iai.73.9.5767-5774.2005
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发表时间：
2005-09-01
影响因子：
3.1
通讯作者：
Heitman, J
中科院分区：
文献类型：
--
作者：
Blankenship, JR;Heitman, J
钙激活蛋白磷酸酶钙调神经磷酸酶在白念珠菌的毒力中起关键作用。以前的研究表明，钙调磷酸酶是不需要酵母菌丝二型转变，宿主细胞粘附，或宿主细胞损伤，这都是建立这种生物体的毒力属性。然而，钙调神经磷酸酶对于血清中的存活和播散性感染是必需的。在这里，我们确定的血清成分是有毒的钙调磷酸酶突变细胞。蛋白质、肽、脂质和其他疏水组分均被排除为必需毒性元素。在测试血清中存在的小分子后，我们发现钙调磷酸酶保护细胞免受血清中存在的内源性钙离子水平引起的应激。这些研究说明了钙调神经磷酸酶如何在钙稳态途径中发挥作用，使普通人类骨骼肌能够通过血流的恶劣环境生存下来，从而建立深层感染并引起疾病。
The calcium-activated protein phosphatase calcineurin plays a critical role in the virulence of Candida albicans. Previous studies demonstrated that calcineurin is not required for the yeast-hypha dimorphic transition, host cell adherence, or host cell injury, which are all established virulence attributes of this organism. Calcineurin is, however, essential for survival in serum and disseminated infection. Here we identify the component of serum that is toxic to calcineurin mutant cells. Proteins, peptides, lipids, and other hydrophobic components were all excluded as essential toxic elements. Upon testing of small molecules present in serum, we discovered that calcineurin protects cells from stress caused by the endogenous levels of calcium ions present in serum. These studies illustrate how calcineurin functions in a calcium homeostatic pathway that enables a common human commensal to survive passage through the hostile environment of the bloodstream to establish deep-seated infections and cause disease.