Combined effects of 50 Hz magnetic field and magnetic nanoparticles on the proliferation and apoptosis of PC12 cells.
Combined effects of 50 Hz magnetic field and magnetic nanoparticles on the proliferation and apoptosis of PC12 cells.
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DOI：
10.3967/bes2014.022
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发表时间：
2014-02
期刊：
影响因子：
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通讯作者：
H. Jia;Chao Wang;Yue Li;Yan Lu;Ping Wang;Wei Pan;T. Song
中科院分区：
文献类型：
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作者：
H. Jia;Chao Wang;Yue Li;Yan Lu;Ping Wang;Wei Pan;T. Song
目的研究极低频磁场（50 Hz，400 μT）和磁性纳米粒子（MNP）对PC 12细胞的细胞毒性和凋亡作用。方法采用透射电镜、动态光散射和电滞回线等方法对SiO2修饰的MNP进行表征。将MNP-SiO2与MF联合或不联合作用于PC 12细胞48 h。MTT法检测细胞毒性，Annexin V-FITC/PI染色检测细胞凋亡。用透射电子显微镜（TEM）测定了MNP-SiO_2的形貌和吸附量。采用Ansoft麦克斯韦软件进行了基于有限元法的MF仿真。结果MNP-SiO_2为直径约20 nm的铁磁性颗粒。MNP-SiO 2以剂量依赖性方式降低细胞活力。MF还随着MNP-SiO 2浓度的增加而降低细胞活力。单独的MNP-SiO_2不能引起PC 12细胞凋亡;相反，在MF暴露和增加剂量的MNP-SiO_2下，凋亡细胞的比例显著增加。MNP-SiO_2可被摄入，然后引起细胞形态的轻微变化。结论MF和MNP-SiO_2联合作用对PC 12细胞有明显的细胞毒性作用，并可促进细胞凋亡。结果表明，MF暴露可以增强MNP的MF，这可能会增强ELF MF的生物效应。
OBJECTIVE To investigate the bioeffects of extremely low frequency (ELF) magnetic field (MF) (50 Hz, 400 μT) and magnetic nanoparticles (MNPs) via cytotoxicity and apoptosis assays on PC12 cells. METHODS MNPs modified by SiO₂ (MNP-SiO₂) were characterized by transmission electron microscopy (TEM), dynamic light scattering and hysteresis loop measurement. PC12 cells were administrated with MNP-SiO2 with or without MF exposure for 48 h. Cytotoxicity and apoptosis were evaluated with MTT assay and annexin V-FITC/PI staining, respectively. The morphology and uptake of MNP-SiO₂ were determined by TEM. MF simulation was performed by Ansoft Maxwell based on the finite element method. RESULTS MNP-SiO₂ were identified as ~20 nm (diameter) ferromagnetic particles. MNP-SiO₂ reduced cell viability in a dose-dependent manner. MF also reduced cell viability with increasing concentrations of MNP-SiO₂. MNP-SiO₂ alone did not cause apoptosis in PC12 cells; instead, the proportion of apoptotic cells increased significantly under MF exposure and increasing doses of MNP-SiO₂. MNP-SiO₂ could be ingested and then cause a slight change in cell morphology. CONCLUSION Combined exposure of MF and MNP-SiO₂ resulted in remarkable cytotoxicity and increased apoptosis in PC12 cells. The results suggested that MF exposure could strengthen the MF of MNPs, which may enhance the bioeffects of ELF MF.