Picomole analysis of glutathione, glutathione disulfide, glutathione S-sulfonate, and cysteine S-sulfonate by high-performance liquid chromatography.
Picomole analysis of glutathione, glutathione disulfide, glutathione S-sulfonate, and cysteine S-sulfonate by high-performance liquid chromatography.
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采用高效液相色谱法对谷胱甘肽、谷胱甘肽二硫化物、谷胱甘肽 S-磺酸盐和半胱氨酸 S-磺酸盐进行皮摩尔分析。
DOI：
10.1016/0003-2697(85)90197-6
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发表时间：
1985
影响因子：
2.9
通讯作者：
Menzel,DB
中科院分区：
文献类型：
--
作者：
Keller,DA;Menzel,DB
建立了同时测定谷胱甘肽、谷胱甘肽二硫化物、谷胱甘肽S磺酸盐和半胱氨酸S磺酸盐的微克分子含量的高效液相色谱方法。化合物用柠檬酸缓冲体系阴离子交换层析分离，然后用邻苯二甲醛和2-巯基乙醇在柱后衍生化，加热到70℃，荧光检测。GSH、CySSO3H、GSSG和GSSO3H的保留时间分别为12.5min、27.5min、29.8min和33.0min，检出限分别为10、200、10和50pmol。GSH的回收率为103%，GSSG的回收率为102%，CySSO3H的回收率为100%，GSSO3H的回收率为96%。描述了细胞中目标化合物的测定。
A method for simultaneous detection of picomole quantities of glutathione (GSH), glutathione disulfide (GSSG), glutathione S-sulfonate (GSSO3H), and cysteine S-sulfonate (CYSSO3H) by high-performance liquid chromatography has been developed. Compounds are separated by anion-exchange chromatography using a citric acid buffer system, and then derivatized postcolumn using o-phthalaldehyde with 2-mercaptoethanol, heated to 70°C, and detected by fluorescence. The compounds elute with retention times of 12.5 min for GSH, 27.5 min for CYSSO3H, 29.8 min for GSSG, and 33.0 minutes for GSSO3H, with detection limits of 10, 200, 10, and 50 pmol, respectively. Recoveries are 103% for GSH, 102% for GSSG, 100% for CYSSO3H, and 96% for GSSO3H. Determination of target compounds in cells is described.