Foxm1 regulates neuronal progenitor fate during spinal cord regeneration
Foxm1 regulates neuronal progenitor fate during spinal cord regeneration
复制标题
Foxm1在脊髓再生过程中调节神经元祖细胞命运
DOI：
10.1101/2020.02.26.962977
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Pelzer D
中科院分区：
文献类型：
--
作者：
Pelzer D
xenopustadpole有能力在截肢后再生它们的尾巴。尽管一些调节尾巴再生的分子和细胞机制已经被确定，但对损伤的组织特异性反应仍然知之甚少。通过对截肢前后分离的脊髓进行大细胞和单细胞RNA测序，研究人员鉴定出在脊髓再生过程中特异性表达的一些基因。我们发现Foxm1，一种已知促进增殖的转录因子，对脊髓再生至关重要。令人惊讶的是，Foxm1并不控制神经祖细胞的细胞周期长度，而是调节它们分裂后的命运。在oxm1 - / -蝌蚪中，我们观察到再生脊髓中的神经元数量减少，这表明神经元分化是再生过程所必需的。总之，我们的数据揭示了脊髓对损伤的特异性反应，并揭示了再生过程中神经元分化的新作用。
Xenopustadpoles have the ability to regenerate their tails upon amputation. Although some of the molecular and cellular mechanisms that globally regulate tail regeneration have been characterised, tissue‐specific response to injury remains poorly understood. Using a combination of bulk and single‐cell RNA sequencing on isolated spinal cords before and after amputation, we identify a number of genes specifically expressed in the spinal cord during regeneration. We show that Foxm1, a transcription factor known to promote proliferation, is essential for spinal cord regeneration. Surprisingly, Foxm1 does not control the cell cycle length of neural progenitors but regulates their fate after division. Infoxm1−/−tadpoles, we observe a reduction in the number of neurons in the regenerating spinal cord, suggesting that neuronal differentiation is necessary for the regenerative process. Altogether, our data uncover a spinal cord‐specific response to injury and reveal a new role for neuronal differentiation during regeneration.
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影响因子：
2.7
作者：
Muñoz R;Edwards-Faret G;Moreno M;Zuñiga N;Cline H;Larraín J
通讯作者：
Larraín J
DOI：
10.1242/dev.019893
发表时间：
2008-06
期刊：
--
影响因子：
--
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影响因子：
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影响因子：
4.6
作者：
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通讯作者：
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