How specific is the first recognition step of homologous recombination?
How specific is the first recognition step of homologous recombination?
复制标题
同源重组的第一个识别步骤有多具体？
DOI：
10.1016/s0968-0004(00)88976-8
复制
发表时间：
1995
影响因子：
13.8
通讯作者：
Radding,CM
中科院分区：
文献类型：
--
作者：
Rao,BJ;Chiu,SK;Bazemore,LR;Reddy,G;Radding,CM
大肠杆菌RecA蛋白通过非沃森-克里克键促进碱基三联体中的同源识别，该非沃森-克里克键不同于由嘌呤或嘧啶组成的DNA非酶促形成的键。碱基替换揭示了识别是宽容的，这与以牺牲精度为代价实现速度的同源性搜索相一致。
TheEscherichia coliRecA protein promotes homologous recognition in base triplets via non-Watson-Crick bonds that differ from those formed nonenzymically from DNA consisting of runs of purines or pyrimidines. Base substitutions reveal recognition to be permissive, consistent with a search for homology that achieves speed at the cost of precision.