Complete nucleotide sequence of the gene for a surface protein antigen of Streptococcus sobrinus
Complete nucleotide sequence of the gene for a surface protein antigen of Streptococcus sobrinus
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远缘链球菌表面蛋白抗原基因的完整核苷酸序列
DOI：
10.1128/iai.59.9.3309-3312.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Toshitaka Koga
中科院分区：
文献类型：
--
作者：
Masayuki Tokuda;N. Okahashi;Ichiro Takahashi;M. Nakai;Shigetaka Nagaoka;Masataka Kawagoe;Toshitaka Koga
测定远缘链球菌MT3791（血清型g）细胞表面蛋白抗原（SpaA）基因的完整核苷酸序列。spaA基因全长4，698 bp，编码170，202 Da的蛋白质，在其氨基末端有一个信号肽。一个潜在的启动子序列和一个假定的Shine-Dalgarno序列位于开放阅读框之前。SpaA中存在两个内部重复氨基酸序列。一个重复区域，位于氨基末端区域，富含丙氨酸，另一个位于中心区域，富含脯氨酸。SpaA的分子结构与变形链球菌表面蛋白抗原的分子结构非常相似。
The complete nucleotide sequence of the gene for a cell surface protein antigen (SpaA) of Streptococcus sobrinus MT3791 (serotype g) was determined. The spaA gene consisted of 4,698 bp and coded for a protein of 170,202 Da. A putative signal peptide was found in the amino-terminal end of the protein. A potential promoter sequence and a putative Shine-Dalgarno sequence preceded the open reading frame. Two internal repeating amino acid sequences were present in SpaA. One repeating region, located in the amino-terminal region, was rich in alanine, and the other, located in the central region, was rich in proline. The molecular structure of SpaA was very similar to that of the surface protein antigen of Streptococcus mutans.