Cloning and sequencing of arg3 and arg11 genes of Schizosaccharomyces pombe on a 10-kb DNA fragment. Heterologous expression and mitochondrial targeting of their translation products.
Cloning and sequencing of arg3 and arg11 genes of Schizosaccharomyces pombe on a 10-kb DNA fragment. Heterologous expression and mitochondrial targeting of their translation products.
复制标题
对 10 kb DNA 片段上的粟酒裂殖酵母 arg3 和 arg11 基因进行克隆和测序。
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
F. Messenguy
中科院分区：
文献类型：
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作者：
C. Huffel;Evelyne Dubois;F. Messenguy
通过功能互补的方法克隆了裂殖酵母精氨酸合成代谢基因arg 3和arg 11。pombe arg 3和arg 11突变株。粟酒酵母DNA基因组文库。两个克隆的限制性内切酶分析和测序表明，这两个基因位于一个共同的DNA片段。arg 3基因编码一个327个氨基酸的多肽，与酿酒酵母和人鸟氨酸氨甲酰基转移酶具有很强的同一性。arg 11基因编码884个氨基酸的多肽。乙酰谷氨酸激酶和乙酰谷氨酸-磷酸还原酶结构域已通过它们与S.酿酒酵母ARG 5，6蛋白。克隆了S.粟酒裂殖酵母不与S. ARG 5，6基因也不与酿酒酵母互补。粟酒酵母arg 11突变。与此相反，这两个鸟氨酸氨甲酰基转移酶编码基因的功能在S。粟酒然而，S.粟酒裂殖酵母arg 3基因与arg 3 S基因的互补性很弱。酿酒酵母菌的表达水平较低，这与酿酒酵母菌的低表达水平一致。粟酒裂殖酵母基因。啤酒。这两种酶的亚细胞定位表明，与S。粟酒裂殖酵母的酶活为95%以上。酿酒酵母酶仍然存在于S.粟酒胞质S.粟酒裂殖酵母中的鸟氨酸氨甲酰基转移酶。cerevisiae不允许其定位。S.粟酒裂殖酵母arg 3和arg 11基因之间以及与S.啤酒。
The Schizosaccharomyces pombe arginine anabolic genes encoding ornithine carbamoyltransferase (arg3) and acetylglutamate kinase/acetylglutamyl-phosphate reductase (arg11) were cloned by functional complementation of S. pombe arg3 and arg11 mutant strains from S. pombe DNA genomic libraries. Restriction analysis and sequencing of the two clones showed that both genes are located on a common DNA fragment. The arg3 gene encodes a 327-amino-acid polypeptide presenting a strong identity to Saccharomyces cerevisiae and human ornithine carbamoyltransferases. The arg11 gene encodes a 884-amino-acid polypeptide. The acetylglutamate kinase and acetylglutamate-phosphate reductase domains have been defined by their identity with the S. cerevisiae ARG5,6 protein. The cloned arg11 gene from S. pombe does not complement an arg5,6 mutation in S. cerevisiae, nor does the ARG5,6 gene complement the S. pombe arg11- mutation. In contrast, both ornithine-carbamoyltransferase-encoding genes function in S. pombe. However, the S. pombe arg3 gene complements only weakly an arg3 S. cerevisiae strain, which is in agreement with the low level of expression of the S. pombe gene in S. cerevisiae. The subcellular localization of both ornithine carbamoyltransferases in the two yeasts indicates that, in contrast to the S. pombe enzyme, more than 95% of the S. cerevisiae enzyme remains in the S. pombe cytoplasm. The low expression of S. pombe ornithine carbamoyltransferases in S. cerevisiae did not allow its localization. The promoters of S. pombe arg3 and arg11 genes do not present striking similarities among themselves nor with the promoters of the equivalent genes of S. cerevisiae.
粗糙脉孢菌中两种可分离线粒体酶的单一前体蛋白。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Wandinger-Ness,AU;Weiss,RL
通讯作者：
Weiss,RL
影响因子：
56.9
作者：
HORWICH, AL;FENTON, WA;ROSENBERG, LE
通讯作者：
ROSENBERG, LE