Molecular phylogeny of a newfound hantavirus in, the Japanese shrew mole (Urotrichus talpoides)
Molecular phylogeny of a newfound hantavirus in, the Japanese shrew mole (Urotrichus talpoides)
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DOI：
10.1073/pnas.0808942105
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发表时间：
2008-10-21
影响因子：
11.1
通讯作者：
Yanagihara, Richard
中科院分区：
文献类型：
--
作者：
Arai, Satoru;Ohdachi, Satoshi D.;Yanagihara, Richard
最近的分子证据表明，在广泛分离的地理区域捕获的遗传上不同的汉坦病毒在鼩 鼱，证实了几十年前的报告，汉坦病毒抗原在鼩 鼱组织。除了挑战啮齿类动物是主要储存宿主的传统观点外，最近发现的soricid-borne汉坦病毒提出了其他soricomorphs，特别是talpids，同样携带汉坦病毒的可能性。在分析2008年2月至4月在日本捕获的日本地鼠（Urotrichus talpoides）肺组织的RNA提取物时，通过RT-PCR检测到汉坦病毒基因组，命名为Asama病毒（ASAV）。配对比对和比较的S-，M-和L-段的核苷酸和氨基酸序列表明，ASAV是遗传更相似的汉坦病毒窝藏的鼩比啮齿动物。然而，预测的ASAV核衣壳蛋白的二级结构是类似的啮齿类动物和地鼠传播的汉坦病毒，表现出相同的卷曲螺旋在氨基末端。系统发育分析，使用最大似然法和其他算法，始终把ASAV与最近确定的soricine 鼩传播的汉坦病毒，表明在遥远的过去可能的主机切换事件。鼠源汉坦病毒的发现扩大了我们对汉坦病毒复杂进化史的认识。
Recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. Apart from challenging the conventional view that rodents are the principal reservoir hosts, the recently identified soricid-borne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. In analyzing RNA extracts from lung tissues of the Japanese shrew mole (Urotrichus talpoides), captured in Japan between February and April 2008, a hantavirus genome, designated Asama virus (ASAV), was detected by RT-PCR. Pairwise alignment and comparison of the S-, M-, and L-segment nucleotide and amino acid sequences indicated that ASAV was genetically more similar to hantaviruses harbored by shrews than by rodents. However, the predicted secondary structure of the ASAV nucleocapsid protein was similar to that of rodent- and shrew-borne hantaviruses, exhibiting the same coiled-coil helix at the amino terminus. Phylogenetic analyses, using the maximum-likelihood method and other algorithms, consistently placed ASAV with recently identified soricine shrew-borne hantaviruses, suggesting a possible host-switching event in the distant past. The discovery of a mole-borne hantavirus enlarges our concepts about the complex evolutionary history of hantaviruses.