Transcriptional repression of euchromatic genes by Drosophila heterochromatin protein 1 and histone modifiers
Transcriptional repression of euchromatic genes by Drosophila heterochromatin protein 1 and histone modifiers
复制标题
DOI：
10.1073/pnas.211303598
复制
发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Worman, HJ
中科院分区：
文献类型：
--
作者：
Hwang, KK;Eissenberg, JC;Worman, HJ
在果蝇中，异染色质蛋白1(HP1)通过将异染色质结构扩展到邻近的常染色质中，抑制人工移位到异染色质附近的常染色质基因的表达。这项研究的目的是确定在没有染色体重排的情况下，HP1是否作为转录抑制因子发挥作用。在这里，我们表明，果蝇HP1通常以剂量依赖的方式抑制四个常染色质基因的表达。受HP1调控的三个基因定位于2号染色体的细胞学区域31，唾液腺中的抗HP1抗体对该区域进行免疫染色。SU(Var)3-9的突变降低了HPI的抑制作用，SU(Var)3-9的哺乳动物同源基因编码组蛋白H3甲基转移酶，SU(Var)2-1突变与组蛋白H4去乙酰化相关。这些数据提供了HP1家族蛋白在后生动物中抑制常染基因表达的遗传学证据，以及组蛋白修饰物与HP1在常染基因抑制中的协同作用。
In Drosophila, heterochromatin protein 1 (HP1) suppresses the expression of euchromatic genes that are artificially translocated adjacent to heterochromatin by expanding heterochromatin structure into neighboring euchromatin. The purpose of this study was to determine whether HP1 functions as a transcriptional repressor in the absence of chromosome rearrangements. Here, we show that Drosophila HP1 normally represses the expression of four euchromatic genes in a dosage-dependent manner. Three genes regulated by HP1 map to cytological region 31 of chromosome 2, which is immunostained by anti-HP1 antibodies in the salivary gland. The repressive effect of HPI is decreased by mutation in Su(var)3-9, whose mammalian orthologue encodes a histone H3 methyltransferase and mutation in Su(var)2-1, which is correlated with histone H4 deacetylation. These data provide genetic evidence that an HP1-family protein represses the expression of euchromatic genes in a metazoan, and that histone modifiers cooperate with HP1 in euchromatic gene repression.