Ocular deficits associated with alcohol exposure during zebrafish development
Ocular deficits associated with alcohol exposure during zebrafish development
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DOI：
10.1002/cne.21320
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发表时间：
2007-06-01
影响因子：
2.5
通讯作者：
Rabin, Richard A.
中科院分区：
文献类型：
--
作者：
Dlugos, Cynthia A.;Rabin, Richard A.
大约90%的胎儿酒精综合症病例伴有眼部异常。斑马鱼（Danio rerio）是一种众所周知的发育模型，它为更好地了解发育暴露于乙醇对脊椎动物眼睛的组织学和细胞学影响提供了机会。本研究的目的是确定斑马鱼模型中乙醇发育暴露的总体、微观和超微结构影响。从 WT 近交系斑马鱼获得卵，并将卵暴露于 0%、0.1%、0.2%、0.4%、0.5% 或 1.0% (v/v) 乙醇中，以评估活力以及剂量和暴露持续时间对眼睛大小的影响。对乙醇处理的幼虫和对照幼虫进行光学和电子显微镜检查。结果表明，乙醇处理使活力在 0.1-0.5% 乙醇浓度下降低约 20%，在 1.0% 乙醇浓度下降低 50%。在受精后 6 天 (dpf) 记录了与乙醇相关的眼睛尺寸减小，并且呈剂量依赖性。与对照幼虫相比，暴露于乙醇的 9 dpf 幼虫的光感受器、内核和神经节层以及晶状体的体积显着减少。超微结构检查显示，与对照组相比，乙醇处理的鱼出现发育滞后的迹象，并且 6 dpf 乙醇处理的幼鱼出现异常视网膜细胞凋亡。这些结果表明，发育中的斑马鱼眼睛对乙醇的干扰敏感，并表现出胎儿酒精综合症中存在的一些眼睛缺陷。
Approximately 90% of fetal alcohol syndrome cases are accompanied by ocular abnormalities. The zebrafish (Danio rerio) is a well-known developmental model that provides an opportunity for better understanding the histological and cytological effects of developmental exposure to ethanol on the vertebrate eye. The purpose of the present study was to determine the gross, microscopic, and ultrastructual effects of developmental exposure to ethanol in the zebrafish model. Eggs were obtained from WT outbred zebrafish and exposed to 0%, 0.1%, 0.2%, 0.4%, 0.5%, or 1.0% (v/v) ethanol to assess viability and the effect of dose and duration of exposure on eye size. Light and electron microscopy were performed on ethanol-treated and control larvae. Results showed that ethanol treatment decreased viability by about 20% at concentrations of 0.1-0.5% ethanol and by 50% at 1.0% ethanol. Ethanol-related decreases in eye size were recorded at 6 days postfertilization (dpf) and were dose dependent. There were significant decreases in the volumes of the photoreceptor, inner nuclear, and ganglionic layers and in the lens of 9 dpf ethanol-exposed compared with control larvae. Ultrastructural examination showed signs of developmental lags in the ethanol-treated fish as well as abnormal retinal apoptosis in the 6 dpf ethanol-treated larvae compared with their controls. These results demonstrate that the developing zebrafish eye is sensitive to perturbation with ethanol and displays some of the eye deficits present in fetal alcohol syndrome.