APOLIPOPROTEIN MULTIGENE FAMILY - TANDEM ORGANIZATION OF HUMAN APOLIPOPROTEIN-A-I, APOLIPOPROTEIN-CIII, AND APOLIPOPROTEIN-AIV GENES
APOLIPOPROTEIN MULTIGENE FAMILY - TANDEM ORGANIZATION OF HUMAN APOLIPOPROTEIN-A-I, APOLIPOPROTEIN-CIII, AND APOLIPOPROTEIN-AIV GENES
复制标题
DOI：
10.1073/pnas.82.19.6374
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
KARATHANASIS, SK
中科院分区：
文献类型：
--
作者：
KARATHANASIS, SK
血浆脂质转运系统的两种蛋白质载脂蛋白AI（apoAI）和CIII（apoCIII）的基因在人类基因组中紧密相连。约30-含有这些基因及其侧翼序列的DNA片段已被克隆和广泛表征。杂交研究表明，位于apoAI基因3“端12 kb的DNA片段含有与人胎儿肠和成人肝脏中1.8 kb mRNA转录物同源的序列，但不包括胎儿肝脏、肾脏、心脏、大脑或肌肉中的序列。将该DNA片段用作探针，从成人肝cDNA文库中分离克隆。该克隆的核苷酸序列与大鼠载脂蛋白AIV（apoAIV）（脂质转运系统的另一种蛋白质）的cDNA序列具有74.8%的同源性，并且编码与大鼠apoAIV具有58.6%相同性的蛋白质。这些结果表明，apoAI，apoCIII和apoAIV基因在人类基因组中紧密相连，并表明它们都来自一个共同的祖先前体。
The genes for two of the proteins of the plasma lipid transport system, apolipoprotein AI (apoAI) and CIII (apoCIII) are closely linked in the human genome. An .apprxeq. 30-kilobase (kb) DNA segment containing these genes and their flanking sequences has been cloned and extensively characterized. Hybridization studies revealed that a DNA fragment located 12 kb 3'' to the apoAI gene contains sequences homologous to a 1.8-kb mRNA transcript in human fetal intestine and adult liver but not in fetal liver, kidney, heart, brain, or muscle. This DNA fragment was used as a probe to isolate a clone from an adult human liver cDNA library. The nucleotide sequence of this clone is 74.8% homologous to the cDNA sequence of rat apolipoprotein AIV (apoAIV), another protein of the lipid transport system, and codes for a protein that is 58.6% identical to rat apoAIV. These results indicate that apoAI, apoCIII, and apoAIV genes are closely linked in the human genome and suggest that all three of them are derived from a common ancestral precursor.