Localization and function of GLUT1 glucose transporter in chicken sperm (Gallus gallus domesticus): relationship between ATP production pathways and flagellar motility.
Localization and function of GLUT1 glucose transporter in chicken sperm (Gallus gallus domesticus): relationship between ATP production pathways and flagellar motility.
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鸡精子（鸡内金）中 GLUT1 葡萄糖转运蛋白的定位和功能：ATP 生产途径与鞭毛运动之间的关系。
DOI：
10.1071/rd19240
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Asano A
中科院分区：
文献类型：
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作者：
Setiawan R;Priyadarshana C;Tajima A;Travis AJ;Asano A
葡萄糖通过糖酵解和氧化磷酸化在精子鞭毛运动和生育力中起重要作用，尽管ATP产生的主要机制在物种之间存在差异。葡萄糖转运蛋白1（glucose transporter 1，GLUT 1）是哺乳动物精子中的一种高亲和力的葡萄糖转运蛋白。然而，在鸟类精子中，葡萄糖代谢途径的特点很差。这项研究表明，GLUT1在葡萄糖介导的鸡精子运动中起着重要作用。使用特异性抗体和配体，我们发现GLUT1特异性定位于中间段。精子活力分析表明，在0 - 80min孵育期间，葡萄糖支持精子运动。然而，这是废除了添加GLUT1抑制剂，伴随着大量减少葡萄糖摄取和ATP的生产，其次是线粒体活性升高响应葡萄糖添加。更有效的抑制ATP的产生和线粒体活性的观察，在响应与氧化磷酸化解偶联剂治疗。由于线粒体抑制只减少了一个子集的精子运动，我们研究了糖酵解途径的本地化，并显示甘油醛-3-磷酸脱氢酶和己糖激酶I的中段和主要部分的鞭毛。本研究的结果提供了新的见解ATP生产途径在鸟类精子的机制。
Glucose plays an important role in sperm flagellar motility and fertility via glycolysis and oxidative phosphorylation, although the primary mechanisms for ATP generation vary between species. The glucose transporter 1 (GLUT1) is a high-affinity isoform and a major glucose transporter in mammalian spermatozoa. However, in avian spermatozoa, the glucose metabolic pathways are poorly characterised. This study demonstrates that GLUT1 plays a major role in glucose-mediated motility of chicken spermatozoa. Using specific antibodies and ligand, we found that GLUT1 was specifically localised to the midpiece. Sperm motility analysis showed that glucose supported sperm movement during incubation for 0–80min. However, this was abolished by the addition of a GLUT1 inhibitor, concomitant with a substantial decrease in glucose uptake and ATP production, followed by elevated mitochondrial activity in response to glucose addition. More potent inhibition of ATP production and mitochondrial activity was observed in response to treatment with uncouplers of oxidative phosphorylation. Because mitochondrial inhibition only reduced a subset of sperm movements, we investigated the localisation of the glycolytic pathway and showed glyceraldehyde-3-phosphate dehydrogenase and hexokinase I at the midpiece and principal piece of the flagellum. The results of this study provide new insights into the mechanisms involved in ATP production pathways in avian spermatozoa.