Cytokine gene expression in chronic periodontitis
Cytokine gene expression in chronic periodontitis
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DOI：
10.1034/j.1600-051x.2001.028009840.x
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发表时间：
2001-09-01
影响因子：
6.7
通讯作者：
Attström, R
中科院分区：
文献类型：
--
作者：
Bickel, M;Axtelius, B;Attström, R
背景：细胞因子在控制炎症过程和组织稳态中起重要作用。牙周炎和其他慢性炎症一样，是宿主因子失调的结果，主要是细胞因子和起始因子。细胞因子的调节不仅受宿主控制，而且受感染细菌及其产物的控制。目的：在本研究中，我们检测了6例患者的细胞因子mRNA表达谱，每个患者的表现部位分别为：(1)严重进行性牙周炎，(2)慢性但稳定的牙周病变，(3)健康部位。定量RT-PCR分析包括ifn - γ、IL-1 β、IL-2、IL-4、IL-5、IL-6和tnf - α。材料与方法：对6例慢性牙周炎患者进行为期6年的治疗，观察局部健康、局部牙周病理但无附着丧失进展迹象和证实进展的部位进行生物化。对活检组织进行分析，分析细胞因子mrna的水平。结果：结果显示，不仅在患者之间，而且在个体部位之间也存在相当大的差异。因此，每个病人的部位都必须被视为一个独立的实体。结论：细胞因子的局部作用严重依赖于免疫活性细胞的募集、相互作用和活化，这可以解释细胞因子表达的位点特异性。来自个体部位的细胞因子数据以及当地临床状况和文献数据表明牙周病发病机制的复杂性。为了深入了解具体的机制，需要进一步的研究。
Background: Cytokines play an important role in controlling inflammatory processes and tissue homeostasis. Periodontitis, as any other chronic inflammatory disease, results from a disarrangement of host factors, mainly cytokines and the initiating agent. Modulation of the cytokines is not only controlled by the host but also by infecting bacteria and their products.Aim: In the present study, we examined the cytokine mRNA expression profiles in six patients, each presenting sites affected with (1) severe progressive periodontitis, (2) chronic, but stable periodontal lesions, and (3) with healthy sites. Analysis using a quantitative RT-PCR included IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-6, and TNF-alpha.Material and Methods: 6 patients with chronic periodontitis were following treatment observed for a period of six years for local sites staying healthy, local sites with periodontal pathology but without signs of progression of attachment loss and sites with verified progression were biosied. The biopsies were lyzed and analyzed for levels of cytokine mRNAs. Results: Results revealed considerable variation not only between patients, but also between individual sites. Each patient's site has thus to be looked at as an independent entity.Conclusions: The local action of cytokines, which is heavily dependent on recruitment, interaction and activation of immunocompetent cells can explain the site-specific nature of cytokine expression. Cytokine data from individual sites together with the local clinical status and data from the literature demonstrate the complexity of periodontal disease pathogenesis. To gain insight to specific mechanisms further studies are needed.