slam encodes a developmental regulator of polarized membrane growth during cleavage of the Drosophila embryo
slam encodes a developmental regulator of polarized membrane growth during cleavage of the Drosophila embryo
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DOI：
10.1016/s1534-5807(02)00141-7
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发表时间：
2002-04-01
影响因子：
11.8
通讯作者：
Wieschaus, E
中科院分区：
文献类型：
--
作者：
Lecuit, T;Samanta, R;Wieschaus, E
果蝇胚胎的细胞化是细胞质分裂的一种特殊形式，它将膜的生长与极化上皮的形成结合在一起。我们已经确定了在细胞化过程中质膜极化生长所必需的基因。在slam突变胚中，沟管被打乱，跨膜蛋白的极化插入被打乱。在细胞化的缓慢阶段，slam蛋白具有显著的发育诱导作用，并定位于沟管和基部连接处。Slam与连接蛋白Arm/ β -catenin、含有PDZ结构域的蛋白Dlt和Myosin共定位，也是它们正确的膜定位所必需的。我们的研究结果表明，Slam的发育诱导通过在粘附连接处募集膜靶向蛋白来组织膜的极化生长。
Cellularization of the Drosophila embryo is a specialized form of cytokinesis that couples membrane growth with the formation of a polarized epithelium. We have identified a gene essential for polarized growth of the plasma membrane during cellularization. In slam mutant embryos, the furrow canal is disorganized, and polarized insertion of transmembrane proteins is disrupted. slam shows a striking developmental induction during the slow phase of cellularization, and Slam protein localizes to the furrow canal and the basal junction. Slam colocalizes with the junctional proteins Arm/beta-catenin, the PDZ domain-containing protein Dlt, and Myosin and is also required for their proper membrane localization. Our results suggest that developmental induction of Slam organizes the polarized growth of membrane via the recruitment of membrane-targeting proteins at adherens junctions.