The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets
The ADP/ATP ratio: A novel predictive assay for quality assessment of isolated pancreatic islets
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DOI：
10.1111/j.1600-6143.2006.01474.x
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发表时间：
2006-10-01
影响因子：
8.8
通讯作者：
Korsgren, O.
中科院分区：
文献类型：
--
作者：
Goto, M.;Holgersson, J.;Korsgren, O.
目前胰岛产品放行标准的标准测定无法预测临床胰岛移植后的结果。因此，建立可靠和快速的检测方法，使胰岛移植前预测的潜力是必要的。在本研究中，我们评估了腺苷二磷酸（ADP）/腺苷三磷酸（ATP）试验，葡萄糖刺激的胰岛素释放，在培养的第一个24小时内胰岛的损失，和胰岛素/脱氧核糖核酸作为预测分析的能力，分离的猪胰岛治愈无胸腺小鼠链脲佐菌素诱导的糖尿病。从所呈现的结果可以得出结论，ADP/ATP比率的测量是与移植结果相关的唯一测试。总之，我们认为ADP/ATP测定法是值得应用于人胰岛移植的，并试图验证它作为移植结果的快速和有效的预测。
The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.