The role of the surface chemistry of CoCr alloy particles in the phagocytosis and DNA damage of fibroblast cells
The role of the surface chemistry of CoCr alloy particles in the phagocytosis and DNA damage of fibroblast cells
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DOI：
10.1002/jbm.a.31064
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发表时间：
2007-08-01
影响因子：
4.9
通讯作者：
Learmonth, I.
中科院分区：
文献类型：
--
作者：
Lewis, A. C.;Ladon, D.;Learmonth, I.
CoCr颗粒的表面化学被证明是体外成纤维细胞吞噬过程的基础。在血清中预孵育5天并在加入细胞培养物之前在水中洗涤的颗粒比在最小必需培养基（MEM）中预孵育1小时并在水中洗涤的颗粒更不容易被吞噬。这是解释了涂层的磷酸钙和蛋白质的血清浸泡颗粒的飞行时间二次离子质谱法研究。与MEM浸泡的颗粒相比，与血清浸泡的颗粒孵育的细胞有丝分裂指数降低，表明吞噬的颗粒导致细胞周期停滞。在颗粒浸入MEM中的第一个小时内通过原子吸收光谱法测量的可溶性离子的释放被鉴定为通过单细胞凝胶电泳（“彗星”测定）测量的DNA损伤的最可能原因。使用具有8 nm空间分辨率的低温聚焦离子束SEM横切细胞，以研究吞噬颗粒的位置，其中一些被发现在核膜内。本文证明，考虑表面化学对于理解骨科磨损碎屑的影响过程至关重要。(c)2007 Wiley Periodicals，Inc. J Biomed Mater Res 82A：363372，2007.
Surface chemistry of CoCr particles is demonstrated to be fundamental to the process of phagocytosis by fibroblast cells hi vitro. Particles preincubated hi serum for 5 days and washed in water before addition to cell cultures were phagocytosed less readily than were particles preincubated in minimal essential medium (MEM) for 1 h and washed in water. This was explained by the coating of calcium phosphate and protein on the serum-immersed particles investigated by time-of-flight secondary ion mass spectroscopy. The cells incubated with the serum-immersed particles had a reduced mitotic index when compared with the MEM-immersed particles, indicating that the phagocytosed particles were causing cell cycle arrest. The release of soluble ions measured by electrothermal atomic absorption spectros- copy within the first hour of particle immersion in MEM was identified as the most likely cause for the DNA damage measured by single cell gel electrophoresis ("Comet" assay). Cryofocused ion beam SEM with a spatial resolution of 8 nm was used to cross section cells, to investigate the location of the phagocytosed particles, some of which were found within the nuclear membrane. This paper demonstrated that consideration of the surface chemistry is essential to understand the processes of the effects of orthopedic wear debris. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res 82A: 363372,2007.