A preliminary study of metalloproteins in CSF by CapLC-ICPMS and NanoLC-CHIP/ITMS.
A preliminary study of metalloproteins in CSF by CapLC-ICPMS and NanoLC-CHIP/ITMS.
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CapLC-ICPMS 和 NanoLC-CHIP/ITMS 对脑脊液中金属蛋白的初步研究。
DOI：
10.1021/pr800024k
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发表时间：
2008
影响因子：
4.4
通讯作者：
Caruso,JosephA
中科院分区：
文献类型：
--
作者：
Ellis,Jenny;DelCastillo,Estela;MontesBayon,Maria;Grimm,Rudolf;Clark,JosephF;Pyne-Geithman,Gail;Wilbur,Steve;Caruso,JosephA
脑脊液（CSF）经常被研究，以探讨总金属浓度在神经退行性疾病患者。神经系统疾病的一些实例包括但不限于脑内出血、脑室内出血、创伤性脑损伤、蛛网膜下腔出血和脑积水。然而，在这项研究中，开始使用金属组学方法进行全面的研究。首先，使用两个分子量截止过滤器按分子量分离CSF组分。然后用毛细管液相色谱法/正孔液相色谱法分离剩余的CSF，并用电感耦合质谱法（ICPMS）进行分析。通过该ICPMS筛选，通过纳米液相色谱-CHIP/离子阱质谱法（nanoLC-CHIP/ITMS）鉴定结合Spectrum Mill数据库搜索提出了一种可能的铁相关蛋白。在这项初步研究中，三种不同类型的合并CSF的部分特征在于其金属（铅，镁，锌，铁和铜）含有的物种与建议进行更全面的研究。CSF和金属成分的化学“差异”表明，该分析对理解蛛网膜下腔出血后观察到的一些并发症具有一定的实用性。
Cerebrospinal fluid (CSF) has frequently been studied to explore the total metal concentrations in patients with neurodegenerative diseases. Some examples of neurologic diseases include but are not limited to intracerebral hemorrhage, intraventricular hemorrhage, traumatic brain injury, subarachnoid hemorrhage and hydrocephalus. In this study, however, a comprehensive approach was begun using metallomics methods. First, two molecular weight cutoff filters were used to separate CSF constituents by molecular weight. The remaining CSF was then separated with capillary liquid chromatography/normal bore liquid chromatography and analyzed with inductively coupled mass spectrometry (ICPMS). With this ICPMS screening, a possible iron associated protein was suggested by nanoliquid chromatography-CHIP/ion trap mass spectrometry (nanoLC-CHIP/ITMS) identification in conjunction with a Spectrum Mill database search. In this preliminary study, three different types of pooled CSF were partially characterized by their metal (Pb, Mg, Zn, Fe and Cu) containing species with suggestions for fuller studies. Chemical ‘differences’ in the CSF and metal constituents suggests some utility in this analysis for understanding some of the complications observed following subarachnoid hemorrhage.