Toward Standardized High-Throughput Serum Diagnostics: Multiplex-Protein Array Identifies IL-8 and VEGF as Serum Markers for Colon Cancer
Toward Standardized High-Throughput Serum Diagnostics: Multiplex-Protein Array Identifies IL-8 and VEGF as Serum Markers for Colon Cancer
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DOI：
10.1177/1087057111414894
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发表时间：
2011-10-01
影响因子：
--
通讯作者：
Habermann, Jens Karsten
中科院分区：
文献类型：
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作者：
Buenger, Stefanie;Haug, Ulrike;Habermann, Jens Karsten
结肠癌的发生、发展可能与细胞因子有关。具有诊断价值的细胞因子已被单独鉴定，但尚未应用于临床实践。使用多重蛋白阵列，作者同时探索了一组细胞因子，并将其性能与癌胚抗原（CEA）和碳水化合物抗原19-9（CA 19-9）进行了比较。采用多重生物芯片技术同时测定50例结肠癌患者和50例健康对照者血清中12种细胞因子的浓度。肿瘤患者血清白细胞介素-8（IL-8）和CEA水平显著高于健康对照组。IL-8的受试者工作特征曲线（AUC）下的面积最大，其次是CEA、血管内皮生长因子（VEGF）和CA 19-9。关于标志物组合的分析显示，CEA、VEGF和CA 19-9优于单一标志物性能，但IL-8没有。多重生物芯片阵列技术代表了一种实用的工具，在细胞因子和癌症的研究时，同时测定不同的生物标志物是感兴趣的。结果表明，IL-8，CEA，VEGF和可能的CA 19-9血清水平的评估可能是有用的结肠癌筛查也检测早期肿瘤的潜力。鼓励进一步验证研究使用这些和其他标记的多重阵列格式。（Journal of Biomolecular Screening 2011;16：1018-1026）
Development and progression of colon cancer may be related to cytokines. Cytokines with diagnostic value have been identified individually but have not been implemented into clinical praxis. Using a multiplex protein array, the authors explore a panel of cytokines simultaneously and compared its performance to carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA 19-9). Serum concentrations of 12 cytokines were simultaneously determined by multiplex biochip technology in 50 colon cancer patients and 50 healthy controls. Serum levels of interleukin-8 (IL-8) and CEA were significantly higher in cancer patients than in healthy controls. Areas under the receiver operating characteristic curves (AUCs) were largest for IL-8, followed by CEA, vascular endothelial growth factor (VEGF), and CA 19-9. Analyses regarding marker combinations showed an advantage over single marker performance for CEA, VEGF, and CA 19-9 but not for IL-8. Multiplex biochip array technology represents a practical tool in cytokine and cancer research when simultaneous determination of different biomarkers is of interest. The results suggest that the assessment of IL-8, CEA, VEGF, and possibly CA 19-9 serum levels could be useful for colon cancer screening with the potential of also detecting early stage tumors. Further validation studies using these and additional markers on a multiplex array format are encouraged. (Journal of Biomolecular Screening 2011;16:1018-1026)