Molecular characterization of Nipah virus, a newly emergent paramyxovirus
Molecular characterization of Nipah virus, a newly emergent paramyxovirus
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DOI：
10.1006/viro.2000.0340
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发表时间：
2000-06-05
期刊：
影响因子：
3.7
通讯作者：
Rota, PA
中科院分区：
文献类型：
--
作者：
Harcourt, BH;Tamin, A;Rota, PA
最近，一种新的副粘病毒（现称为尼帕病毒（NV））在马来西亚和新加坡出现，导致人类致命脑炎和猪呼吸道综合征。初步研究表明，NV与亨德拉病毒（HV）在抗原性和遗传学上相关。我们生成了NV的N、P/C/V、M、F和G基因的序列，并将这些序列与HV和副粘病毒科其他成员的序列进行了比较。NV与HV的基因间隔区完全相同，基因起始和终止序列也几乎完全相同。在NV中发现了P基因中V和C蛋白的开放阅读框（ORF），但HV P基因中编码潜在的短碱性蛋白的ORF在NV中不保守。与HV相比，NV的N、P、C、V、M、F和G ORF的核苷酸同源性为88%至70%，预测的氨基酸同源性为92%至67%。与HV相比，NV的F蛋白的预测融合切割序列具有单个氨基酸取代（K至R）。系统发育分析表明，虽然HV和NV密切相关，但它们明显不同于副粘病毒科中任何已建立的属，应被视为一个新属，（C）2000学术出版社。
Recently, a new paramyxovirus, now known as Nipah virus (NV), emerged in Malaysia and Singapore, causing fatal encephalitis in humans and a respiratory syndrome in pigs. Initial studies had indicated that NV is antigenically and genetically related to Hendra virus (HV). We generated the sequences of the N, P/C/V, M, F, and G genes of NV and compared these sequences with those of HV and other members of the family Paramyxoviridae. The intergenic regions of NV were identical to those of HV, and the gene start and stop sequences of NV were nearly identical to those of HV. The open reading frames (ORFs) for the V and C proteins within the P gene were found in NV, but the ORF encoding a potential short basic protein found in the P gene of HV was not conserved in NV. The N, P, C,V, M, F, and G ORFs in NV have nucleotide homologies ranging from 88% to 70% and predicted amino acid homologies ranging from 92% to 67% in comparison with HV. The predicted fusion cleavage sequence of the F protein of NV had a single amino acid substitution (K to R) in comparison with HV. Phylogenetic analysis demonstrated that although HV and NV are closely related, they are clearly distinct from any of the established genera within the Paramyxoviridae and should be considered a new genus, (C) 2000 Academic Press.