Heterologous Expression of AtWRKY57 Confers Drought Tolerance in Oryza sativa.
Heterologous Expression of AtWRKY57 Confers Drought Tolerance in Oryza sativa.
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AtWRKY57 的异源表达赋予水稻耐旱性
DOI：
10.3389/fpls.2016.00145
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yu D
中科院分区：
文献类型：
--
作者：
Jiang Y;Qiu Y;Hu Y;Yu D
干旱胁迫是严重限制植物分布和作物生产的严重环境因素。最近，我们发现过表达AtWRKY57可以增强拟南芥的耐旱性。在这项研究中，我们进一步报道了拟南芥WRKY57转录因子能够赋予转基因水稻（Oryza sativa）植物耐旱性。转基因水稻的耐旱性增强是由于在胁迫条件下较低的失水率、细胞死亡、丙二醛含量和相对电解质泄漏，同时观察到较高的脯氨酸含量和活性氧清除酶活性。此外，进一步的研究表明，与野生型植物相比，耐旱转基因水稻植物中一些应激反应基因的表达水平上调。除了耐旱性外，AtWRKY57 过表达植物还具有增强的盐和 PEG 胁迫耐受性。综上所述，我们的研究表明，在水稻中过度表达 AtWRKY57 不仅可以提高耐旱性，还可以提高耐盐性和 PEG 耐受性，这证明了其在作物改良中的潜在作用。
Drought stress is a severe environmental factor that greatly restricts plant distribution and crop production. Recently, we have found that overexpressing AtWRKY57 enhanced drought tolerance in Arabidopsis thaliana. In this study, we further reported that the Arabidopsis WRKY57 transcription factor was able to confer drought tolerance to transgenic rice (Oryza sativa) plants. The enhanced drought tolerance of transgenic rice was resulted from the lower water loss rates, cell death, malondialdehyde contents and relative electrolyte leakage while a higher proline content and reactive oxygen species-scavenging enzyme activities was observed during stress conditions. Moreover, further investigation revealed that the expression levels of several stress-responsive genes were up-regulated in drought-tolerant transgenic rice plants, compared with those in wild-type plants. In addition to the drought tolerance, the AtWRKY57 over-expressing plants also had enhanced salt and PEG stress tolerances. Taken together, our study indicates that over-expressing AtWRKY57 in rice improved not only drought tolerance but also salt and PEG tolerance, demonstrating its potential role in crop improvement.