Antagonistic Effect of Short Neuropeptide F on Allatotropin-Inhibited Feeding Motivation of the Silkworm Larva, Bombyx mori
Antagonistic Effect of Short Neuropeptide F on Allatotropin-Inhibited Feeding Motivation of the Silkworm Larva, Bombyx mori
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DOI：
10.2108/zs180119
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发表时间：
2019-02
影响因子：
0.9
通讯作者：
Sumihiro Matsumoto;A. Ohara;Chiaki Nagai-Okatani;Yi-jun Zhou;Daiki Fujinaga;Hitomi Seike;S. Nagata
中科院分区：
文献类型：
--
作者：
Sumihiro Matsumoto;A. Ohara;Chiaki Nagai-Okatani;Yi-jun Zhou;Daiki Fujinaga;Hitomi Seike;S. Nagata
在本研究中，我们证实了短神经肽F(SNPF)对家蚕摄食动力的拮抗作用；sNPF可减少抑制肽--促生长素(AT)的延缓摄食作用。原位杂交和MALDI-TOF MS分析表明，在中肠内分泌细胞中存在三种sNPF亚型(sNPF-1、-2和-3)。钙离子成像分析表明，sNPF受体的三种亚型(BNGR-A7、-A10和-A11)与这三种亚型的sNPF具有不同的亲和力。此外，sNPF通过ERK在中肠的磷酸化激活其信号，而sNPF和AT的混合物降低了磷酸化水平，这与行为学实验结果一致。综上所述，我们目前的发现表明，肠道sNPF通过减少AT在中肠内的抑制作用，积极地调节了摄食动机。
Here, we demonstrated an antagonistic effect of short neuropeptide F (sNPF) in modulating feeding motivation in the silkworm Bombyx mori; sNPF reduced the feeding-delaying effects caused by administration of an inhibitory peptide, allatotropin (AT). In situ hybridization and MALDI-TOF MS analysis revealed the presence of three subtypes of sNPFs (sNPF-1, -2, and -3) in the midgut enteroendocrine cells. Ca2+-imaging analyses revealed that three subtypes of sNPF receptors (sNPFRs) (BNGR-A7, -A10, and -A11) showed different affinities with the three subtypes of sNPFs. In addition, sNPF activated its signaling via ERK phosphorylation in the midgut, while mixture of sNPF and AT reduced the phosphorylation level, agreeing with the results of behavioral assay. Together, our current findings suggest that intestinal sNPF positively modulates the feeding motivation by reducing the inhibitory effects by AT within the midgut.