Atomic force microscopy study of early morphological changes during apoptosis
Atomic force microscopy study of early morphological changes during apoptosis
复制标题
DOI：
10.1021/la051837g
复制
发表时间：
2005-09-27
期刊：
影响因子：
3.9
通讯作者：
Baker, J
中科院分区：
文献类型：
--
作者：
Hessler, JA;Budor, A;Baker, J
细胞凋亡是由细胞死亡过程中观察到的一系列独特的形态变化来定义的，包括局部粘连的丧失，细胞膜芽或气泡的形成，以及总细胞体积的减少。最近的研究表明，这些显著的形态变化，特别是凋亡体积减少(AVD)，是细胞凋亡的早期先决条件，并先于关键的生化时间点。在这里，我们使用原子力显微镜来观察星形孢子素诱导的KB细胞凋亡的早期AVD。在1亩M星形孢子素作用3小时后，观察到总细胞高度下降32%，总细胞体积损失50%，同时细胞直径只有15%的变化。观察到的AVD先于细胞凋亡的关键生化特征，如线粒体膜电位丧失、磷脂酰丝氨酸转位、核碎裂和可测量的caspase-3活性。这表明形态体积的变化在诱导细胞凋亡的早期就发生了。
Apoptosis is defined by a distinct set of morphological changes observed during cell death including loss of focal adhesions, the formation of cell membrane buds or blebs, and a decrease in total cell volume. Recent studies suggest that these dramatic morphological changes, particularly apoptotic volume decrease (AVD), are an early prerequisite to apoptosis and precede key biochemical time-points. Here we use atomic force microscopy to observe early stage AVD of KB cells undergoing staurosporine-induced apoptosis. After a 3-h exposure to 1 mu M staurosporine, a 32% decrease in total cell height and a 50% loss of total cell volume is observed accompanied by only a 15% change in cell diameter. The observed AVD precedes key biochemical hallmarks of apoptosis such as loss of mitochondrial membrane potential, phosphatidyl serine translocation, nuclear fragmentation, and measurable caspase-3 activity. This suggests that morphological volume changes occur very early in the induction of apoptosis.