Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
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DOI：
10.1111/cei.12021
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发表时间：
2013-03-01
影响因子：
4.6
通讯作者：
Katoh, N.
中科院分区：
文献类型：
--
作者：
Sano, Y.;Masuda, K.;Katoh, N.
胸腺基质淋巴细胞生成素（TSLP）以其诱导CD 11c+髓样树突状细胞促进辅助性T细胞2型（Th2）偏态炎症反应的能力而闻名。虽然TSLP的表达增加的特应性皮炎（AD）患者的皮损中的有限数量的报道，TSLP的表达程度和AD的严重程度，表皮屏障功能和皮疹类型之间的关系仍有待阐明。本研究的目的是在相当大的一组患者中使用非侵入性方法检查皮肤中TSLP表达程度与AD严重程度、皮疹类型和表皮屏障功能之间的关系。通过胶带剥离从AD患者获得角质层组织，并使用TSLP特异性抗体评价角质层TSLP（scTSLP）表达水平，随后进行图像分析。分析了scTSLP强度和AD严重程度评分（SCORAD）指数与表皮屏障功能（如角质层水合作用和经表皮水分丢失（TEWL））之间的相关性。还检查了由保湿剂的应用诱导的scTSLP水平的变化。与健康受试者相比，AD患者的scTSLP表达水平增加，并与SCORAD相关，特别是与皮肤干燥评分和角质层水合作用相关。施用脱硫剂导致scTSLP水平降低。scTSLP水平可用作AD严重程度和特别是表皮屏障状态的生物标志物。
Thymic stromal lymphopoietin (TSLP) is known for its capacity to induce CD11c+ myeloid dendritic cells to promote T helper type 2 (Th2)-skewed inflammatory responses. Although increased expression of TSLP was reported in the lesional skin of limited numbers of patients with atopic dermatitis (AD), the relationships between the degree of TSLP expression in the skin and the severity of AD, epidermal barrier function and eruption type remain to be elucidated. The aim of this study was to examine the relationships between the degree of TSLP expression in the skin and the severity of AD, eruption type and epidermal barrier function using a non-invasive method in a sizeable group of the patients. Stratum corneum tissue was obtained from AD patients by tape stripping, and the stratum corneum TSLP (scTSLP) expression level was evaluated using a TSLP-specific antibody followed by image analysis. The correlations between the scTSLP intensity and the severity scoring of AD (SCORAD) index and epidermal barrier function, such as stratum corneum hydration and transepidermal water loss (TEWL), were analysed. The changes in the scTSLP level induced by the application of moisturizer were also examined. The scTSLP expression level was increased in AD patients compared with healthy subjects and was correlated with SCORAD, especially with the dry skin score, and stratum corneum hydration. Moisturizer application resulted in reduced scTSLP levels. The scTSLP level can be used as a biomarker of AD severity and particularly epidermal barrier status.