Xoom is maternally stored and functions as a transmembrane protein for gastrulation movement in Xenopus embryos
Xoom is maternally stored and functions as a transmembrane protein for gastrulation movement in Xenopus embryos
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Xoom 是母体储存的，并作为非洲爪蟾胚胎原肠胚形成运动的跨膜蛋白
DOI：
10.1046/j.1440-169x.2001.00549.x
复制
发表时间：
2001
期刊：
影响因子：
4.6
通讯作者：
T. Kinoshita
中科院分区：
文献类型：
--
作者：
Kouichi Hasegawa;Nobuko Sakurai;T. Kinoshita
Xoom是一个在非洲爪蟾胚胎原肠胚形成过程中起重要作用的新基因。虽然Xoom在卵子发生过程中被积极转录，但其翻译产物的分布和功能尚未明确。本研究制备了针对Xoom的多克隆抗体，以研究Xoom蛋白的行为。抗Xoom抗体显示爪蟾胚胎中存在两种形式的Xoom蛋白：(i) 45 kDa可溶性细胞质形式；（ii） 44 kDa的膜相关形式。两种形式的Xoom蛋白在未受精卵至蝌蚪期普遍存在，在囊胚期和原胚期达到定性高峰。免疫组织化学检查显示，Xoom蛋白原体储存在动物皮层下层，并在卵裂阶段分裂为外胚层细胞。酶解膜蛋白和Xoom的免疫检测表明Xoom作为膜相关蛋白存在。为了检测Xoom蛋白的功能，将抗Xoom抗体注射到7期囊胚胚胎的囊胚精中。抗Xoom抗体以剂量依赖性的方式引起原肠胚发育缺陷。这些结果表明母体制备的Xoom蛋白参与了外胚层细胞的原肠胚形成运动。
Xoom has been identified as a novel gene that plays an important role in gastrulation of Xenopus laevis embryo. Although Xoom is actively transcribed during oogenesis, distribution and function of its translation product have not yet been clarified. In the present study, the polyclonal antibody raised against Xoom was generated to investigate a behavior of Xoom protein. Anti‐Xoom antibodies revealed that there are two forms of Xoom protein in Xenopus embryos: (i) a 45 kDa soluble cytoplasmic form; and (ii) a 44 kDa membrane‐associated form. Two forms of Xoom protein were ubiquitously detected from unfertilized egg to tadpole stage, with a qualitative peak during blastula and gastrula stages. Immunohistochemical examination showed that Xoom protein is maternally stored in the animal subcortical layer and divided into presumptive ectodermal cells during cleavage stages. Enzymatic digestion of membrane protein and immunologic detection of Xoom showed that Xoom exists as a membrane‐associated protein. To examine a function of Xoom protein, anti‐Xoom antibodies were injected into blastocoele of stage 7 blastula embryo. Anti‐Xoom antibodies caused gastrulation defect in a dose‐ dependent manner. These results suggest that maternally prepared Xoom protein is involved in gastrulation movement on ectodermal cells.
整合素依赖性粘附活性在原肠胚形成时由感应信号在空间上控制。
DOI：
10.1242/dev.122.9.2873
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ramos,JW;Whittaker,CA;DeSimone,DW
通讯作者：
DeSimone,DW
非洲爪蟾原肠胚形成过程中瓶细胞的行为和功能。
DOI：
10.1242/dev.103.1.211
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hardin,J;Keller,R
通讯作者：
Keller,R
影响因子：
2.7
作者：
Winklbauer, R;Keller, RE
通讯作者：
Keller, RE