Persistence of a repressed Epstein-Barr virus genome in Burkitt lymphoma cells made resistant to 5-bromodeoxyuridine.
Persistence of a repressed Epstein-Barr virus genome in Burkitt lymphoma cells made resistant to 5-bromodeoxyuridine.
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伯基特淋巴瘤细胞中持续存在的受抑制的 Epstein-Barr 病毒基因组对 5-溴脱氧尿苷产生了耐药性。
DOI：
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发表时间：
1971
影响因子：
11.1
通讯作者：
J. Walker
中科院分区：
文献类型：
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作者：
B. Hampar;J. G. Derge;L. Martos;J. Walker
对EB病毒阳性的人淋巴母细胞系P3HR-1进行了5-溴脱氧尿嘧啶核苷抗性试验。在5-溴脱氧尿苷维持的P3HR-1(BU)细胞中产生Epstein-Barr病毒相关抗原，但不产生病毒颗粒。然而，在药物移除后的4天内，确实出现了病毒颗粒。胸苷激酶活性仅限于产生病毒抗原的P3HR-1(BU)细胞，而所有对照P3HR-1细胞均显示胸苷激酶活性，而与病毒抗原合成无关。大多数P3HR-1(BU)细胞中的细胞DNA是通过不涉及胸苷激酶的途径制造的。在具有胸苷激酶途径的细胞中，检测到第二个密度为1.71g/cm(3)的DNA，对应于Epstein-Barr病毒。结论是：(A)不含胸苷激酶的P3HR-1(BU)细胞中持续存在抑制的Epstein-Barr病毒基因组，病毒基因组的激活伴随着生产性感染和酶的出现，以及(B)P3HR-1(BU)细胞中的胸苷激酶活性可作为病毒基因组表达的标志。
The P3HR-1 line of human lymphoblastoid cells that is Epstein-Barr virus positive was made resistant to 5-bromodeoxyuridine. Epstein-Barr virus-associated antigens, but not virus particles, were produced in P3HR-1(BU) cells maintained on 5-bromodeoxyuridine. However, virus particles did appear within 4 days after removal of the drug. Thymidine kinase activity was limited to P3HR-1(BU) cells producing viral antigen, whereas all control P3HR-1 cells showed thymidine kinase activity regardless of viral antigen synthesis. Cellular DNA in most P3HR-1(BU) cells was made via pathways that did not involve thymidine kinase. In cells having a pathway that involved thymidine kinase, a second DNA of density 1.71 g/cm(3), corresponding to Epstein-Barr virus, was detected.IT WAS CONCLUDED THAT: (a) a repressed Epstein-Barr virus genome persists in P3HR-1(BU) cells that do not contain thymidine kinase, with activation of the viral genome being accompanied by productive infection and the appearance of enzyme, and (b) thymidine kinase activity in P3HR-1(BU) cells could be used as a marker for viral genome expression.