PknE, a serine/threonine protein kinase from Mycobacterium tuberculosis has a role in adaptive responses
PknE, a serine/threonine protein kinase from Mycobacterium tuberculosis has a role in adaptive responses
复制标题
DOI：
10.1007/s00203-012-0848-4
复制
发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Narayanan, Sujatha
中科院分区：
文献类型：
--
作者：
Kumar, Dinesh;Palaniyandi, Kannan;Narayanan, Sujatha
丝氨酸/苏氨酸蛋白激酶（STPK）在结核分枝杆菌的生理和发病机制中起主要作用。在这里，我们研究了pknE，一个STPK在适应性反应的M。使用缺失突变Delta pknE的结核病。在应激（pH、表面活性剂和细胞壁损伤剂）和抗结核药物的存在下评估Delta pknE的存活。Δ pknE在pH 7.0和溶菌酶（一种细胞壁破坏剂）中生长不良，在pH 5.5、SDS和卡那霉素（一种二线抗结核药物）中存活较好。此外，Delta pknE在液体培养基中生长期间细胞大小减小，并且在豚鼠感染模型中表现出超强毒力。结论：pknE可能参与了巨噬细胞的适应性反应.结核病调节细胞的完整性和存活。
Serine/threonine protein kinases (STPK) play a major role in the physiology and pathogenesis of Mycobacterium tuberculosis. Here, we have examined the role of pknE, a STPK in the adaptive responses of M. tuberculosis using a deletion mutant Delta pknE. The survival of Delta pknE was assessed in the presence of stress (pH, surfactant and cell wall-damaging agents) and anti-tuberculosis drugs. Delta pknE had a defective growth in pH 7.0 and lysozyme (a cell wall-damaging agent) with better survival in pH 5.5, SDS and kanamycin (a second-line anti-tuberculosis drug). Furthermore, Delta pknE was reduced in cell size during growth in liquid media and exhibited hypervirulence in a guinea pig model of infection. In conclusion, our data suggest that pknE plays a role in adaptive response of M. tuberculosis regulating cellular integrity and survival.