Fungal metabolites. XIX. Structural elucidation of channel-forming peptides, trichorovins-I-XIV, from the fungus Trichoderma viride.
Fungal metabolites. XIX. Structural elucidation of channel-forming peptides, trichorovins-I-XIV, from the fungus Trichoderma viride.
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真菌代谢物。
DOI：
10.1248/cpb.43.910
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发表时间：
1995
影响因子：
1.7
通讯作者：
T. Fujita
中科院分区：
文献类型：
--
作者：
S. Wada;A. Iida;N. Akimoto;M. Kanai;N. Toyama;T. Fujita
曲霉菌素(Trichorovins，TV)-I-XIV是从绿色木霉分生孢子中分离得到的新型抗菌肽。TVS的多肽混合物通过制备高效液相反复分级，直到单个TVS在其分析高效液相色谱图上显示单一峰。然而，FAB-MS或核磁共振表明，每个TVS-I-XIV至少由两个组分组成。我们尝试通过电喷雾电离(ESI)-MS、FAB-MS、FAB-MS/MS和核磁共振等手段来确定它们的结构。TVS通常具有大约1100-1200 Da的分子质量，其特征是N-末端乙酰化，C-末端存在氨基醇，例如亮氨醇、异亮氨醇或缬氨醇，分子中有11个残基，包括3个α-氨基异丁酸。因此，确定电视属于多糖类。
Trichorovins (TV)-I-XIV are new antibiotic peptides obtained from conidia of the fungus Trichoderma viride. The peptide mixture of TVs was repeatedly fractionated by preparative HPLC until individual TVs showed a single peak on their analytical HPLC chromatograms. Nevertheless, FAB-MS or NMR indicated that each of TVs-I-XIV was composed of at least two components. We attempted to elucidate their structures within the fractions by electrospray ionization (ESI)-MS, FAB-MS, FAB-MS/MS and NMR. TVs generally have molecular weights of approximately 1100-1200 Da, and are characterized by an acetylated N-terminus, the presence of an aminoalcohol, e.g. leucinol, isoleucinol or valinol, at the C-terminus, and eleven residues including three alpha-aminoisobutyric acids in the molecule. Thus, it was determined that TVs belong to the class of peptaibols.