Visualization of aging-associated chromatin alterations with an engineered TALE system.
Visualization of aging-associated chromatin alterations with an engineered TALE system.
复制标题
使用工程 TALE 系统可视化与衰老相关的染色质改变
DOI：
10.1038/cr.2017.18
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发表时间：
2017-04
期刊：
影响因子：
44.1
通讯作者：
Liu GH
中科院分区：
文献类型：
--
作者：
Ren R;Deng L;Xue Y;Suzuki K;Zhang W;Yu Y;Wu J;Sun L;Gong X;Luan H;Yang F;Ju Z;Ren X;Wang S;Tang H;Geng L;Zhang W;Li J;Qiao J;Xu T;Qu J;Liu GH
未修饰的TALE不足以使四种肿瘤细胞系中的着丝粒可视化。(A)EGFP-TALE centro（绿色）和着丝粒FISH（红色）信号在U2 OS、HeLa、HepG 2和MCF 7细胞中的共定位分析。EGFP-TALE centro使用19-bp着丝粒DNA序列设计。虚线表示核边界;箭头表示重叠信号。比例尺，5 mm。（B）显示所示细胞系的细胞核中着丝粒FISH或EGFP-TALE中心（19 bp）阳性点的数目的直方图。n = 50个核/线。(C)分别在U2 OS和HeLa细胞中EGFP-TALE端粒（绿色）和核仁素（红色，IF）之间不存在共定位。虚线表示核边界。比例尺，5 mm。（D）分别在U2 OS和HeLa细胞中EGFP-TALE中心（绿色）和核仁素（红色，IF）之间不存在共定位。虚线表示核边界。刻度尺，5 mm。
Unmodified TALEs were insufficient to visualize centromeres in four tumor cell lines. (A) Co-localization analysis of EGFP-TALE centro (green) and centromeric FISH (red) signals in U2OS, HeLa, HepG2, and MCF7 cells. EGFP-TALE centro was designed using a 19-bp centromeric DNA sequence. Dashed lines indicate the nuclear boundary; arrowheads indicate overlapping signals. Scale bars, 5 mm. (B) Histograms showing numbers of centromeric FISH-or EGFP-TALE centro (19 bp)-positive dots in nuclei of indicated cell lines. n = 50 nuclei per line. (C) The absence of co-localization between EGFP-TALE telo (green) and nucleolin (red, IF) in U2OS and HeLa cells, respectively. Dashed lines indicate the nuclear boundary. Scale bars, 5 mm. (D) The absence of co-localization between EGFP-TALE centro (green) and nucleolin (red, IF) in U2OS and HeLa cells, respectively. Dashed lines indicate the nuclear boundary. Scale bars, 5 mm.