Defining protective responses to pathogens: cytokine profiles in leprosy lesions.
Defining protective responses to pathogens: cytokine profiles in leprosy lesions.
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定义对病原体的保护性反应：麻风病灶中的细胞因子谱。
DOI：
10.1126/science.254.5029.277
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Modlin,RL
中科院分区：
文献类型：
--
作者：
Yamamura,M;Uyemura,K;Deans,RJ;Weinberg,K;Rea,TH;Bloom,BR;Modlin,RL
在少数人类感染性疾病中，产生耐药性所需的免疫学机制已被确定，而特异性细胞因子的作用尚不清楚。麻风病在临床上表现为一个谱，其中抗性与对病原体的细胞介导的免疫相关。为了评估原位细胞因子模式，从麻风皮肤活检标本中提取的信使RNA通过聚合酶链反应与14个丝氨酸特异性引物扩增。在耐药型疾病的病变中，编码白细胞介素-2和干扰素-γ的信使RNA最明显。相比之下，白细胞介素-4、白细胞介素-5和白细胞介素-10的信使RNA在多杆菌形式中占主导地位。因此，抗性和易感性与细胞因子产生的不同模式相关。
The immunological mechanisms required to engender resistance have been defined in few infectious diseases of man, and the role of specific cytokines is unclear. Leprosy presents clinically as a spectrum in which resistance correlates with cell-mediated immunity to the pathogen. To assess in situ cytokine patterns, messenger RNA extracted from leprosy skin biopsy specimens was amplified by the polymerase chain reaction with 14 cytokine-specific primers. In lesions of the resistant form of the disease, messenger RNAs coding for interleukin-2 and interferon-gamma were most evident. In contrast, messenger RNAs for interleukin-4, interleukin-5, and interleukin-10 predominated in the multibacillary form. Thus, resistance and susceptibility were correlated with distinct patterns of cytokine production.