Specific Targeting of the Basolateral Amygdala to Projectionally Defined Pyramidal Neurons in Prelimbic and Infralimbic Cortex.
Specific Targeting of the Basolateral Amygdala to Projectionally Defined Pyramidal Neurons in Prelimbic and Infralimbic Cortex.
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DOI：
10.1523/eneuro.0002-16.2016
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发表时间：
2016-03
期刊：
影响因子：
3.4
通讯作者：
Sheets PL
中科院分区：
文献类型：
--
作者：
Cheriyan J;Kaushik MK;Ferreira AN;Sheets PL
内侧前额叶皮层的相邻边缘前区（PL）和边缘下区（IL）在情绪学习中有不同的作用。这种二分法背后的完整的机械理解仍然不清楚。在这里，我们探讨了特定的PL和IL神经元的基底外侧杏仁核（BLA），边缘系统的结构在疼痛和恐惧处理的关键。在小鼠中，我们使用逆行标记，脑切片记录和腺病毒光遗传学来解剖上行BLA输入到PL和IL神经元投射到导水管周围灰质（PAG）或杏仁核的连接。我们发现了BLA投射到PL和IL皮质的差异靶向。激活BLA投射在PL和IL皮质的第5层（L5）的皮质PAG（CP）神经元中引起兴奋和抑制反应。然而，所有的抑制反应都是多突触的，单突触的BLA输入对IL皮层的CP神经元更强。相反，BLA优先靶向PL第2层（L2）中的皮质杏仁核（CA）神经元，而不是IL皮质。我们还发现，BLA输入是投影特异性的，显示优先靶向L5 CP神经元在相邻的L3/5 CA神经元在IL皮质。我们的结论表明，BLA输入是椎板特异性的，产生更强的兴奋性反应CA神经元在L3/5相比，L2在IL皮质。总的来说，这项研究揭示了BLA对PL和IL皮质的不同靶向，这取决于皮质神经元的层位置和投射靶点。总的来说，我们的研究结果应该有重要的意义，了解疼痛和恐惧输入的PL和IL皮层的处理。
Adjacent prelimbic (PL) and infralimbic (IL) regions in the medial prefrontal cortex have distinct roles in emotional learning. A complete mechanistic understanding underlying this dichotomy remains unclear. Here we explored targeting of specific PL and IL neurons by the basolateral amygdala (BLA), a limbic structure pivotal in pain and fear processing. In mice, we used retrograde labeling, brain-slice recordings, and adenoviral optogenetics to dissect connectivity of ascending BLA input onto PL and IL neurons projecting to the periaqueductal gray (PAG) or the amygdala. We found differential targeting of BLA projections to PL and IL cortex. Activating BLA projections evoked excitatory and inhibitory responses in cortico-PAG (CP) neurons in layer 5 (L5) of both PL and IL cortex. However, all inhibitory responses were polysynaptic and monosynaptic BLA input was stronger to CP neurons in IL cortex. Conversely, the BLA preferentially targeted corticoamygdalar (CA) neurons in layer 2 (L2) of PL over IL cortex. We also reveal that BLA input is projection specific by showing preferential targeting of L5 CP neurons over neighboring L3/5 CA neurons in IL cortex. We conclude by showing that BLA input is laminar-specific by producing stronger excitatory responses CA neurons in L3/5 compared with L2 in IL cortex. Collectively, this study reveals differential targeting of the BLA to PL and IL cortex, which depends both on laminar location and projection target of cortical neurons. Overall, our findings should have important implications for understanding the processing of pain and fear input by the PL and IL cortex.