A new method of selecting Schwann cells from adult mouse sciatic nerve
A new method of selecting Schwann cells from adult mouse sciatic nerve
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DOI：
10.1016/j.jneumeth.2005.05.004
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发表时间：
2005-11-30
影响因子：
3
通讯作者：
Balian, G
中科院分区：
文献类型：
--
作者：
Pannunzio, ME;Jou, IM;Balian, G
我们描述了一种使用层粘连蛋白筛选和纯化体外雪旺细胞的方法。我们还研究了所选细胞悬浮在海藻酸珠中的体外和体内活力。我们观察到，随着每一轮层粘连蛋白的选择，雪旺细胞培养的均匀性增加，五次传代后达到85-90%。在藻酸珠体内孵育后，细胞的存活率在73%至76%之间，而在单层培养中维持的细胞存活率大于90%。这种使用层粘连蛋白包被表面的连续选择新方法优化了从小鼠成年坐骨神经细胞扩增的雪旺细胞培养物的纯化。这种方法的优点是在技术上比其他方法更容易，并且在雪旺细胞培养中达到80-90%的均匀性。(c) 2005 Elsevier b.v.版权所有
We describe a method of using laminin for the selection and purification of Schwann cells in vitro. We also studied the viability of the selected cells suspended in alginate beads both in vitro and in vivo. We observed that the homogeneity of the Schwann cell culture increased with each round of laminin selection and reached 85-90% after five passages. The viability of cells after incubation within an alginate bead in vivo was between 73 and 76% compared with greater than 90% viability for cells that were maintained in monolayer culture. This new method of serial selection using laminin-coated surfaces has optimized the purification of a Schwann cell culture expanded from cells harvested from the adult sciatic nerve of a mouse. This method has the advantage of being technically easier than other methods described and results in a Schwann cell culture that is 80-90% homogenous. (c) 2005 Elsevier B.V All rights reserved.