Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120
Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120
复制标题
异囊形成多细胞蓝藻鱼腥藻中的时空基因抑制系统。
DOI：
10.1021/acssynbio.8b00496
复制
发表时间：
2019
影响因子：
4.7
通讯作者：
Ehira Shigeki
中科院分区：
文献类型：
--
作者：
Higo Akiyoshi;Ehira Shigeki
形成异囊的多细胞蓝藻。pcc7120常被用作原核细胞分化的模式生物。我们最近证明，杂种囊由于其活跃的分解代谢和微氧条件，适合于光合作用产生有价值的化学物质，如乙醇。我们已经开发了基因调控系统，包括细胞类型特异性基因诱导系统，以扩大这种蓝藻的使用。本研究将细胞类型特异性基因诱导系统与CRISPRi技术相结合，成功构建了异囊特异性条件基因抑制系统。我们以编码谷氨酰胺合成酶的glnagene为目标，重建多细胞蓝藻的代谢，谷氨酰胺合成酶是氮同化的必需酶。异囊特异性抑制glna增强乙醇生产。我们认为，杂种囊特异性基因抑制系统是开展细胞分化基础研究和杂种囊代谢工程的有效工具。
The heterocyst-forming multicellular cyanobacteriumAnabaenasp. PCC 7120 is often used as a model organism for prokaryotic cell differentiation. We recently demonstrated that heterocysts are suitable for photosynthetic production of valuable chemicals, such as ethanol, due to their active catabolism and microoxic conditions. We have developed gene regulation systems, including cell type-specific gene induction systems, to broaden this cyanobacterium’s use. In the present study, a heterocyst-specific conditional gene repression system was successfully created by combining a cell type-specific gene induction system with CRISPRi technology. We targeted theglnAgene that encodes glutamine synthetase, an essential enzyme for nitrogen assimilation, to reconstruct metabolism in the multicellular cyanobacterium. Heterocyst-specific repression ofglnAenhanced ethanol production. We believe that heterocyst-specific gene repression systems are useful tools for basic research on cell differentiation as well as for metabolic engineering of heterocysts.