Transcriptome and enzyme activity analyses of tolerance mechanisms in pearl oyster (Pinctada fucata) under high-temperature stress
Transcriptome and enzyme activity analyses of tolerance mechanisms in pearl oyster (Pinctada fucata) under high-temperature stress
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DOI：
10.1016/j.aquaculture.2022.737888
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发表时间：
2022-01
期刊：
影响因子：
4.5
通讯作者：
Hua Zhang;Huixia Jia;Panpan Xiong;Gaoyou Yao;Maoxian He
中科院分区：
文献类型：
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作者：
Hua Zhang;Huixia Jia;Panpan Xiong;Gaoyou Yao;Maoxian He
合浦珠母贝（Pinctada fucata）是一种具有重要经济价值的海水珍珠养殖贝类。然而，随着全球气候变暖和海水温度上升，许多珍珠牡蛎正在死亡。因此，培育耐高温菌株迫在眉睫。我们通过转录组分析来揭示P. fucatato长期高温胁迫。纳米孔测序鉴定了1787个位点，并揭示了17，803种新型剪接异构体的高质量全长异构体。这项大规模研究表明了热应激下选择性剪接（AS）调节和转录调节的不同模式。高温组（HT）的AS发生率高于常温对照组（NT）。总的来说，44个上调和33个下调的差异表达基因（DEG）之间的NT和HT是明显的。基因本体论（GO）和京都基因与基因组百科全书（KEGG）的途径分析表明，DEG主要与热应激下的防御机制、吞噬体信号转导、细胞骨架、翻译后修饰、蛋白质周转和分子伴侣等相关。9个DEG（c-fox，heat shock protein 60，heat shock protein 90，fucolectin-1，dermatopontin，caveolin-1，ETS homologous factor，glutathioneS-transferase sigma 3和fatty acyl-CoA hydrolase eprecursor）在高温诱导1h至30 d内表达模式发生了复杂的变化。此外，热胁迫导致不规则的壳微观结构和影响酶的活性，例证乳酸脱氢酶（LDH）的持续上调，表明能量需求的P。通过厌氧代谢部分地满足了岩藻毒素。综合分析表明，长期高温应激可显著影响机体内部功能，破坏生理和生化过程。高温胁迫诱导珍珠牡蛎激活基因表达，从而改变免疫反应，呼吸代谢，抗氧化系统，生物矿化和次生代谢。这些途径可能协调以支持P。高温胁迫下的岩藻生理这一发现加深了我们对长期高温胁迫反应的分子机制的理解，并为今后的耐热性育种提供有价值的信息。fucatastrains。
Pearl oyster (Pinctada fucata) is an economically valuable shellfish for seawater pearl production. However, many pearl oysters are dying as the global climate warms and seawater temperatures rise. The breeding of high temperature-resistant strains is therefore urgent. We performed transcriptome analysis to reveal the response mechanism ofP. fucatato long-term high-temperature stress. Nanopore sequencing identified 1787 loci and revealed high-quality full-length isoforms for 17,803 novel splice isoforms. The large-scale study suggested differential patterns of alternative splicing (AS) regulation and transcriptional regulation under heat stress. There were more AS events in the high-temperature group (HT) than the normal temperature (NT) control group. In total, 44 upregulated and 33 downregulated differentially expressed genes (DEGs) were evident between NT and HT. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that DEGs were mainly associated with defence mechanisms, phagosome signalling, cytoskeleton, posttranslational modification, protein turnover and chaperones under heat stress. Nine DEGs (c-fox,heat shock protein60,heat shock protein 90,fucolectin-1,dermatopontin,caveolin-1,ETS homologous factor,glutathioneS-transferase sigma 3andfatty acyl-CoAhydrolaseprecursor) showed complex expression pattern changes from 1 h to 30 days of high-temperature induction. Furthermore, heat stress resulted in irregular shell microstructures and impacted enzyme activity, exemplified by continuous upregulation of lactate dehydrogenase (LDH), indicating that the energy needs ofP. fucataneeds were met in part by anaerobic metabolism. Integrated analyses demonstrated that long-term high-temperature stress can significantly impact internal bodily functions, and disrupt physiological and biochemical processes. High-temperature stress induces pearl oysters to activate gene expression and thereby alter immune responses, respiratory metabolism, antioxidant systems, biomineralisation, and secondary metabolism. These pathways likely coordinate to supportP. fucataphysiology under high-temperature stress. The findings deepen our understanding of the molecular mechanisms underlying the responses to long-term high-temperature stress, and provide valuable information for future breeding of thermotolerantP. fucatastrains.