Characterization of two monoclonal antibodies specific for lacto-series type 1 chain Gal beta 1----3GlcNAc-terminal structures.
Characterization of two monoclonal antibodies specific for lacto-series type 1 chain Gal beta 1----3GlcNAc-terminal structures.
复制标题
对乳系列 1 型链 Gal beta 1----3GlcNAc 末端结构具有特异性的两种单克隆抗体的表征。
DOI：
10.1016/0003-9861(90)90567-i
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发表时间：
1990
影响因子：
3.9
通讯作者：
Greene,TG
中科院分区：
文献类型：
--
作者：
Holmes,EH;Greene,TG
通过用含有末端Galβ1→3GlcNAc结构的生物合成反应产物免疫产生的鼠单克隆抗体TE-1和TE-3已经产生，并发现其与未衍生化的1型链乳糖系列碳水化合物结构特异性反应。对这些抗体（均为IgM）的详细分析表明两种不同类别的表位特异性。发现抗体TE-1优先结合含有末端Galβ1→3GlcNAc二糖的较长链碳水化合物结构，表明最佳抗体结合不仅仅涉及识别该二糖。相反，发现抗体TE-3与含有末端Galβ1→3GlcNAc结构的碳水化合物结构强烈结合，而与链长无关。通过添加岩藻糖和/或唾液酸残基修饰核心链结构完全消除了抗体与任一抗体的结合。从多种人结肠腺癌细胞系分离的中性糖脂的TLC免疫染色表明强烈染色的条带，特别是用抗体TE-3，其与基于1型链的糖脂衍生物的表达水平相关。这些抗体用于详细研究乳糖系列1型链基碳水化合物结构合成的调节。
Murine monoclonal antibodies, TE-1 and TE-3, generated by immunization with a biosynthetic reaction product containing a terminal Galβ1→3GlcNAc structure have been produced and found to react specifically with underivatized type 1 chain lacto-series carbohydrate structures. Detailed analysis of these antibodies, both IgM, indicates two differing classes of epitope specificity. Antibody TE-1 was found to bind preferentially to longer chain carbohydrate structures containing a terminal Galβ1→3GlcNAc disaccharide, indicating that optimal antibody binding involved more than recognition of this disaccharide. In contrast, antibody TE-3 was found to bind strongly carbohydrate structures containing terminal Galβ1→3GlcNAc structures irrespective of chain length. Modification of core chain structures by addition of fucose and/or sialic acid residues completely abolished antibody binding with either antibody. TLC immunostaining of neutral glycolipids isolated from a variety of human colonic adenocarcinoma cell lines indicated intensely stained bands, particularly with antibody TE-3, which correlated with the level of expression of type 1 chain based glycolipid derivatives. These antibodies are applied to the detailed study of the regulation of synthesis of lacto-series type 1 chain based carbohydrate structures.